Biodegradable and versatile polyethylenimine derivatives efficiently transfer DNA and siRNA into mammalian cells

Colloids and Surfaces. B, Biointerfaces
Ruby BansalPradeep Kumar

Abstract

Polyethylenimines (PEIs) are considered as the most promising vectors for non-viral gene delivery applications. Here, we report the synthesis and in vitro evaluation of two non-toxic and biodegradable polymers, TEPA@bPEI (TBP) and TEPA@lPEI (TLP), derived from low molecular weight branched and linear polyethylenimines by the stepwise reactions with methylacrylate (aza-Michael reaction) and amidation with tetraethylenepentamine (TEPA). These polymers not only showed their ability to bind and condense pDNA into nano-sized complexes but also provided protection against nucleases in cellular milieu. Both the polymers exhibited excellent buffering capacity and efficiently delivered nucleic acids (plasmid DNA and siRNA) across the mammalian cells (CHO and A549 cells) and outclassed native polymers and the commercial transfection reagents in terms of transfection efficiency and target gene silencing, and that too without compromising on biocompatibility i.e. The results advocate the promising potential of the PEI derivatives as safe and potent nucleic acid carriers for practical gene delivery applications.

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Citations

Nov 29, 2015·Colloids and Surfaces. B, Biointerfaces·Hui WangJianru Xiao
Jan 21, 2017·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Azadeh Hashem NiaMohammad Ramezani
Feb 22, 2017·Journal of Biomedical Materials Research. Part a·Selgai HaidariAndreas Kolk
Apr 1, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Qiang LiuZhi-Gang Zhao
Aug 8, 2018·Organic & Biomolecular Chemistry·Yong-Guang GaoAi-Rong Qian
Oct 26, 2018·Journal of Biomedical Materials Research. Part a·Andreas KolkChristian Plank
Apr 20, 2018·Drug Delivery and Translational Research·Ruby BansalKailash Chand Gupta
Oct 20, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·T J ThomasC K S Pillai

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