Biodegradable chitin conduit tubulation combined with bone marrow mesenchymal stem cell transplantation for treatment of spinal cord injury by reducing glial scar and cavity formation

Neural Regeneration Research
Feng XueNa Han

Abstract

We examined the restorative effect of modified biodegradable chitin conduits in combination with bone marrow mesenchymal stem cell transplantation after right spinal cord hemisection injury. Immunohistochemical staining revealed that biological conduit sleeve bridging reduced glial scar formation and spinal muscular atrophy after spinal cord hemisection. Bone marrow mesenchymal stem cells survived and proliferated after transplantation in vivo, and differentiated into cells double-positive for S100 (Schwann cell marker) and glial fibrillary acidic protein (glial cell marker) at 8 weeks. Retrograde tracing showed that more nerve fibers had grown through the injured spinal cord at 14 weeks after combination therapy than either treatment alone. Our findings indicate that a biological conduit combined with bone marrow mesenchymal stem cell transplantation effectively prevented scar formation and provided a favorable local microenvironment for the proliferation, migration and differentiation of bone marrow mesenchymal stem cells in the spinal cord, thus promoting restoration following spinal cord hemisection injury.

References

Feb 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yang D TengEvan Y Snyder
Jul 10, 2003·Lancet Neurology·Michael Chopp, Yi Li
Oct 18, 2008·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Peixun ZhangBaoguo Jiang
Feb 24, 2009·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Peixun ZhangBaoguo Jiang
Aug 4, 2010·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Peixun ZhangBaoguo Jiang
Sep 11, 2014·Neural Regeneration Research·Sufang LiuFeng Tao

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Citations

Jan 28, 2016·Regenerative Biomaterials·Liliana R Pires, Ana P Pêgo
Feb 10, 2017·ACS Applied Materials & Interfaces·Qingqing WangJian Xiao
Jan 24, 2017·Journal of Tissue Engineering and Regenerative Medicine·Moonhang KimByung Hyune Choi
Feb 10, 2021·Cell Transplantation·Liyi HuangQuan Wei
Dec 16, 2020·ACS Biomaterials Science & Engineering·Walaa A AbbasNageh K Allam

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Methods Mentioned

BETA
transmission electron microscopy
transgenic
light microscopy
fluorescence microscopy

Software Mentioned

SPSS

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