Biodegradable nanosyringes for intracellular amplification-based dual-diagnosis and gene therapy in single living cells

Chemical Science
Xiaotong ShenNa Na

Abstract

The efficient delivery of biomolecules into living cells as well as their easy biodegradation have been challenges for the application of intracellular amplification for sensitive multiple-diagnosis and gene therapy for cancer. Herein, new strategies of amplification-based dual-detection of cancer biomarkers (Let-7a miRNA and VEGF) and gene therapy for cancers are put forward. These are achieved through biodegradable nanosyringes (NSs), rigid and sharp in vitro but degradable in vivo, which are applied for the efficient loading, delivery and release of biomolecules (enzymes, nucleic acids, and even silencing RNA) into living cells. After penetrating cell membranes and escaping from endosomes through their rigid and sharp tips, NSs release biomolecules for fast and easy "one-step" rolling circle amplification (ring formation and amplification) in single living cells. Therefore, based on signals from two probes, FAM-Probe and Cy5-Probe, that selectively bind to amplification products, 100 aM of Let-7a and 100 fM of VEGF could be detected, which are much lower than reported values. Furthermore, siRNAs can also be delivered by NSs for gene therapy, and their therapeutic effect was evaluated by their in vivo antitumor efficacy in CC...Continue Reading

References

Nov 4, 1998·Nucleic Acids Research·J BanérU Landegren
Oct 31, 2003·Cancer Treatment Reviews·Roy A Patchell
Jan 23, 2004·Nature Reviews. Cancer·John Condeelis, Jeffrey E Segall
Feb 24, 2009·Nature Materials·Ji-Ho ParkMichael J Sailor
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Alex K ShalekHongkun Park
Jul 1, 2011·Nanoscale·Feng-Yun WangJohnny C Ho
Oct 18, 2011·Cell·Scott Valastyan, Robert A Weinberg
Jul 4, 2012·Analytical Chemistry·Ronit FreemanItamar Willner
May 12, 2015·Cancer Cell International·Kaladhar B Reddy
Oct 9, 2015·Nature Communications·Costanza GiampietroClaudia Ghigna
Nov 25, 2016·Nature Communications·Kewei RenHuangxian Ju
Jun 7, 2017·Chemical Society Reviews·Shahed BehzadiMorteza Mahmoudi
Aug 30, 2017·Chemical Society Reviews·Jia WenShiguo Sun
Aug 30, 2018·Accounts of Chemical Research·Jesús MosqueraLuis M Liz-Marzán

❮ Previous
Next ❯

Datasets Mentioned

BETA
M32977

Methods Mentioned

BETA
transmission electron microscopy
dynamic light scattering
X-ray
electrophoresis
xenograft

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.