Biodegradable thermosensitive injectable PEG-PCL-PEG hydrogel for bFGF antigen delivery to improve humoral immunity

Growth Factors
Changyang GongZhiyong Qian

Abstract

In this contribution, a biodegradable and injectable thermosensitive poly(ethylene glycol)-poly(epsilon-caprolactone)-poly(ethylene glycol) (PEG-PCL-PEG, PECE) hydrogel system was successfully prepared for basic fibroblastic growth factor (bFGF) antigen delivery. bFGF encapsulated PECE hydrogel system (bFGF-hydrogel) is an injectable free-flowing sol at ambient temperature, and forms a non-flowing gel at physiological temperature acting as antigen depot. Furthermore, the cytotoxicity results showed that the PECE hydrogel could be regarded as a safe carrier, and bFGF could be released from the hydrogel system in an extended period in vitro. Otherwise, the immunogenicity of bFGF was improved significantly after encapsulated into the hydrogel. Strong humoral immunity created by bFGF-hydrogel was maintained for more than 14 weeks. Therefore, the prepared bFGF loaded PECE hydrogel might have great potential as a novel vaccine adjuvant for protein antigen.

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Citations

Aug 20, 2010·Journal of the Royal Society, Interface·Kangwon LeeDavid J Mooney
Mar 7, 2014·Therapeutic Delivery·Ashaben PatelAshim K Mitra
Feb 20, 2014·Drug Delivery·Bita TaghizadehZaynab Derakhshani
Feb 9, 2011·Acta Biomaterialia·Vivek Kumar GarripelliSeongbong Jo
Dec 9, 2014·Acta Biomaterialia·Justin R AdamsSurya K Mallapragada
Jun 21, 2014·International Journal of Pharmaceutics·Maja Radivojša MatanovićPegi Ahlin Grabnar

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