Biodegradation behavior of silk fibroin membranes in repairing tympanic membrane perforations

Journal of Biomedical Materials Research. Part a
Ok Joo LeeChan Hum Park

Abstract

Silk fibroin (SF) from silkworms has been widely studied as a biomaterial. The degradation behavior of silk biomaterials is important for medical applications, but few studies have examined long-term degradation behavior in vivo. In this study, we investigated the degradation behavior of SF membranes in vitro and in vivo. For the in vitro assay, we observed degradation of silk membranes in phosphate buffered saline, culture media, and an enzyme (proteinase K) solution. In the proteinase K solution, 80% of the silk membranes degraded within 10 days. Silk membranes exhibited no cytotoxicity toward L929 cells and rat tissues. To investigate the degradation of silk membranes in vivo, they were implanted subcutaneously in rats and harvested 19 months after surgery. Scanning electron microscopy imaging and histological analysis of silk membrane explants showed that they broke into several pieces after 16 months. Results show that silk membranes are biocompatible and display excellent long-term degradation behavior when used as biomaterials.

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Citations

Jan 10, 2016·International Journal of Biological Macromolecules·Soo Hyeon KimChan Hum Park
Sep 4, 2015·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Shih-Han HungHow Tseng
Mar 11, 2017·Scientific Reports·Chae Eun KimJaeWook Yang
Oct 11, 2017·Journal of Biomaterials Applications·Ye Been SeoChan Hum Park
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Oct 31, 2021·Nature Protocols·Soon Hee KimChan Hum Park

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