Biodegradation of 2,4-Dichlorophenol through a Distal meta-Fission Pathway.

Applied and Environmental Microbiology
S KohD D Focht

Abstract

Alcaligenes eutrophus JMP222, a derivative of A. eutrophus JMP134 which has lost plasmid pJP4 (encoding the tfd genes for the ortho fission pathway), was induced for the meta fission pathway when grown on o-cresol. Resting cell suspensions, grown on o-cresol, oxidized 2,4-dichlorophenol (2,4-DCP), a degradation product of 2,4-dichlorophenoxyacetic acid, to 3,5-dichlorocatechol. Further degradation of 3,5-dichlorocatechol was observed by the production of a yellow ring fission product with liberation of chloride. Oxidation of 2,4-DCP (305 (mu)M) in 47 hs resulted in 69% dehalogenation through this pathway. The ring fission product was characterized as 2-hydroxy-3,5-dichloro-6-oxo-hexa-2,4-dienoic acid by gas chromatography-mass spectrometry and gas chromatography-Fourier transform infrared spectroscopy. These data indicate that 2,4-DCP is degraded through a distal meta ring fission pathway, in contrast to either a suicidal proximal fission or the standard ortho fission pathway.

References

Mar 1, 1979·Applied and Environmental Microbiology·J HartmannH J Knackmuss
Feb 1, 1979·Nature·W Reineke, H J Knackmuss
Aug 1, 1992·Applied and Environmental Microbiology·F K Higson, D D Focht
Sep 1, 1992·Applied and Environmental Microbiology·P M Radehaus, S K Schmidt
Dec 1, 1992·Applied and Environmental Microbiology·W E HolbenJ M Tiedje
May 1, 1987·Applied and Environmental Microbiology·J G SteiertR L Crawford
Mar 1, 1970·Journal of Agricultural and Food Chemistry·J M DuxburyJ E Dawson
Dec 1, 1982·Applied and Environmental Microbiology·G J Stanlake, R K Finn
May 1, 1980·Journal of Bacteriology·W Reineke, H J Knackmuss
Apr 1, 1996·Applied and Environmental Microbiology·R R FulthorpeJ M Tiedje
Mar 1, 1984·Applied and Environmental Microbiology·I BartelsW Reineke

❮ Previous
Next ❯

Citations

Mar 17, 2012·Environmental Science and Pollution Research International·Nyuk-Min ChongShiu-Ching Tsai
Aug 21, 2015·Environmental Toxicology and Chemistry·Tadeusz PaszkoIzabella Jackowska
Jan 31, 2004·Environmental Science & Technology·Lotte A ReitzelPoul L Bjerg

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.