Biodegradation of a phenolic mixture in a solid-liquid two-phase partitioning bioreactor

Applied Microbiology and Biotechnology
George P Prpich, Andrew J Daugulis

Abstract

A solid-liquid two-phase partitioning bioreactor (TPPB) in which the non-aqueous phase consisted of polymer (HYTREL) beads was used to degrade a model mixture of phenols [phenol, o-cresol, and 4-chlorophenol (4CP)] by a microbial consortium. In one set of experiments, high concentrations (850 mg l(-1) of each of the three substrates) were reduced to sub-inhibitory levels within 45 min by the addition of the polymer beads, followed by inoculation and rapid (8 h) consumption of the total phenolics loading. In a second set of experiments, the beneficial effect of using polymer beads to launch a fermentation inhibited by high substrate concentrations was demonstrated by adding 1,300 and 2,000 mg l(-1) total substrates (equal concentrations of each phenolic) to a pre-inoculated bioreactor. At these levels, no cell growth and no degradation were observed; however, after adding polymer beads to the systems, the ensuing reduced substrate concentrations permitted complete destruction of the target molecules, demonstrating the essential role played by the polymer sequestering phase when applied to systems facing inhibitory substrate concentrations. In addition to establishing alternative modes of TPPB operation, the present work has demo...Continue Reading

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Citations

Sep 13, 2008·Applied Microbiology and Biotechnology·M Concetta TomeiAndrew J Daugulis
Mar 29, 2012·Bioprocess and Biosystems Engineering·Hala Fam, Andrew J Daugulis
Sep 30, 2006·Biotechnology Letters·George P PrpichAndrew J Daugulis
Jun 4, 2013·Applied and Environmental Microbiology·Richard VillemurEric Déziel
May 3, 2011·Journal of Environmental Management·F J Alvarez-HornosC Gabaldón
May 15, 2008·Biotechnology and Bioengineering·Jenna L E MorrishAndrew J Daugulis
Feb 6, 2009·Biotechnology Progress·Andrew J Daugulis
Mar 1, 2012·Critical Reviews in Biotechnology·Sonia MalikMercedes Bonfill
Apr 28, 2007·Biotechnology and Bioengineering·George P Prpich, Andrew J Daugulis
Oct 12, 2007·Biotechnology and Bioengineering·Lars Rehmann, Andrew J Daugulis
Oct 8, 2009·Environmental Science & Technology·M Concetta TomeiAndrew J Daugulis

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