Biodegradation of all stereoisomers of the EDTA substitute iminodisuccinate by Agrobacterium tumefaciens BY6 requires an epimerase and a stereoselective C-N lyase

Applied and Environmental Microbiology
Zeljko CokesaPaul-Gerhard Rieger

Abstract

Biodegradation tests according to Organization for Economic Cooperation and Development standard 301F (manometric respirometry test) with technical iminodisuccinate (IDS) revealed ready biodegradability for all stereoisomers of IDS. The IDS-degrading strain Agrobacterium tumefaciens BY6 was isolated from activated sludge. The strain was able to grow on each IDS isomer as well as on Fe(2+)-, Mg(2+)-, and Ca(2+)-IDS complexes as the sole carbon, nitrogen, and energy source. In contrast, biodegradation of and growth on Mn(2+)-IDS were rather scant and very slow on Cu(2+)-IDS. Growth and turnover experiments with A. tumefaciens BY6 indicated that the isomer R,S-IDS is the preferred substrate. The IDS-degrading enzyme system isolated from this organism consists of an IDS-epimerase and a C-N lyase. The C-N lyase is stereospecific for the cleavage of R,S-IDS, generating d-aspartic acid and fumaric acid. The decisive enzyme for S,S-IDS and R,R-IDS degradation is the epimerase. It transforms S,S-IDS and R,R-IDS into R,S-IDS. Both enzymes do not require any cofactors. The two enzymes were purified and characterized, and the N-termini were sequenced. The purified lyase and also the epimerase catalyzed the transformation of alkaline earth ...Continue Reading

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Citations

Mar 7, 2013·Environmental Science and Pollution Research International·Dorota Kołodyńska
Aug 2, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Bettina BäuerlePaul Gerhard Rieger
Apr 7, 2006·Applied and Environmental Microbiology·Bettina BäuerlePaul-Gerhard Rieger
Aug 15, 2014·Environmental Science and Pollution Research International·Qing WuJianhui Sun
Aug 29, 2006·Journal of Molecular Biology·Bernhard LohkampGunter Schneider
May 29, 2007·Chemical Reviews·R S BoethlingDavid DiFiore

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