Biodegradation of chitosan-tripolyphosphate beads: in vitro and in vivo studies

Artificial Cells, Blood Substitutes, and Immobilization Biotechnology
Serap DurkutA Eser Elçin

Abstract

In this study, chitosan [(1 --> 4) linked 2-amino-2-deoxy-beta-D-glucopyranose] beads were prepared by interacting this polycation (> 90% deacetylated) with the tripolyphosphate (TPP) polyanion. The resulting chitosan-TPP beads (C) were modified either by coating with sodium alginate (CA) or by cross-linking with glutaraldehyde (CGA). The in vitro degradation of C beads was found to be faster than its CA and CGA counterparts. C beads degraded faster at pH 6.5, compared to pH 7.4 conditions. At pH 7.4, about 41%, 37% and 10% of dry mass loss after 12 months was determined for C, CA and CGA, respectively. At pH 6.5, the dry mass loss of CA and CGA after the same period of time was found to be 73% and 37%, respectively. However, C beads completely degraded at pH 6.5 after 8 months of in vitro incubation. The in vivo biodegradation experiments were performed on Wistar rats (n = 24) for a duration of 6 months. No sign of fibrotic capsule formation was observed around any of the implanted beads at 2 and 6 months post-transplantation. At 2 months, the in vivo-degradation was slow-going and the beads in all groups were intact; CGA beads had more tissue reaction than C and CA beads at this time point. While the C beads had almost comple...Continue Reading

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Citations

Jul 12, 2012·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Mitesh Nagar, Adhikrao V Yadav
Jul 5, 2006·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·A Eser Elçin
Aug 26, 2010·Journal of Pharmaceutical Sciences·Jing Wen LohLee-Yong Lim
Apr 11, 2009·Biotechnology Journal·Pinar ZorlutunaVasif Hasirci
Mar 6, 2007·Journal of Biomedical Materials Research. Part a·Bülend InançY Murat Elçin
May 15, 2015·Artificial Cells, Nanomedicine, and Biotechnology·Aysel KoçYaşar Murat Elçin
May 20, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Carlos David Grande TovarJosé Herminsul Mina Hernandez
Mar 28, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·George Z KyzasNikolaos K Lazaridis
Jun 10, 2010·Journal of Agricultural and Food Chemistry·Venkateshwarlu GudipatiEric Andrew Decker

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