Biodegradation of chlorpyrifos and its hydrolysis product 3,5,6-trichloro-2-pyridinol using a novel bacterium Ochrobactrum sp. JAS2: A proposal of its metabolic pathway

Pesticide Biochemistry and Physiology
J Abraham, S Silambarasan

Abstract

Biodegradation of chlorpyrifos and its major metabolite 3,5,6-trichloro-2-pyridinol (TCP) were studied with a novel bacterial strain JAS2 isolated from paddy rhizosphere soil. The molecular characterization based on 16S rRNA gene sequence homology confirmed its identity as Ochrobactrum sp. JAS2. The JAS2 strain degraded 300mgl(-1) of chlorpyrifos within 12h of incubation in the aqueous medium and it produced the TCP metabolite. However, after 72h of incubation TCP was also completely degraded by the JAS2 strain. A tentative degradation pathway of chlorpyrifos by Ochrobactrum sp. JAS2 has been proposed on basis of GC-MS analysis. The complete degradation of chlorpyrifos occurred within 24h in the soil spiked with and without addition of nutrients inoculated with Ochrobactrum sp. JAS2. TCP was obtained in both the studies which was degraded completely by 96h in the soil spiked with nutrients and whereas 120h in absence of nutrients in the soil. The mpd gene which is responsible for organophosphorus hydrolase production was identified. The isolates Ochrobactrum sp. JAS2 also exhibited a time dependent increase in the amount of tricalcium phosphate solubilization in Pikovskaya's medium. Further screening of the strain JAS2 for auxi...Continue Reading

References

Mar 1, 1996·Canadian Journal of Microbiology·C L Patten, B R Glick
Apr 14, 2000·Archives of Microbiology·C Blumer, D Haas
Sep 26, 2001·Applied and Environmental Microbiology·C ZhongliF Guoping
Feb 1, 1991·Applied and Environmental Microbiology·J M BricS E Silverstone
Jan 1, 1951·Plant Physiology·S A Gordon, R P Weber
Jul 5, 2007·Biotechnology Letters·Gangming XuYanchun Yan
Dec 17, 2008·Journal of Hazardous Materials·P C Abhilash, Nandita Singh
Oct 1, 2009·Acta Microbiologica Et Immunologica Hungarica·A ZaidiM Oves
Apr 6, 2011·Environmental Science and Pollution Research International·D P SinghA Gulati
Jul 19, 2012·World Journal of Microbiology & Biotechnology·P N Bhattacharyya, D K Jha

❮ Previous
Next ❯

Citations

Dec 1, 2017·Environmental Science and Pollution Research International·Adolfo Marican, Esteban F Durán-Lara
Nov 24, 2019·International Journal of Environmental Research and Public Health·Taozhong ShiRimao Hua
May 4, 2017·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·David Tirado-TorresMartha Gayosso-Canales
Jul 23, 2020·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Xiaowen SunHong Ni
Jun 27, 2020·Environmental Science and Pollution Research International·Sivagnanam SilambarasanVelu Rajesh Kannan
Nov 19, 2019·Journal of Agricultural and Food Chemistry·Taozhong ShiRimao Hua
Jan 3, 2021·Environmental Research·Yaohua HuangShaohua Chen
Apr 11, 2021·Journal of Hazardous Materials·Pengqiang DuYongquan Zheng
Apr 22, 2020·Environmental Science and Pollution Research International·Tanita PescatoreAnna Barra Caracciolo
Aug 11, 2021·Environmental Science and Pollution Research International·Saliha AhmadChristos A Damalas
Dec 28, 2021·AMB Express·Victoria Conde-AvilaLuis Daniel Ortega Martínez

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioremediation (ASM)

Bioremediation is the treatment and removal of harmful pollutants or contaminants through the use of microorganisms. Discover the latest research here.