Biodegradation of cyanide in cassava wastewater using a novel thermodynamically-stable immobilized rhodanese.

Preparative Biochemistry & Biotechnology
Adedeji Nelson AdemakinwaOladapo Fagbohun

Abstract

Extracellular rhodanese obtained from Aureobasidium pullulans was employed in both free and immobilized forms for the biodegradation of cyanide present in cassava processing mill effluent (CPME). Crosslinking with glutaraldehyde (at an optimum concentration of 5% v/v) before entrapment in alginate beads resulted in the highest immobilization yield of 94.5% and reduced enzyme leakage of 1.8%. Rhodanese immobilized by cross-linking before entrapment (cbe) retained about 46% of its initial activity after eight cycles of catalysis compared to the entrapment in alginate alone (eaa) which lost more than 79% after the fifth catalytic cycle. A cross-examination of thermodynamic (ΔGd*, ΔSd*, ΔHd*) kinetic (kd, t1/2, D and z-values) parameters at 30-70 °C showed that cbe displayed a higher resistance to thermal inactivation when compared to the free enzyme (fe) and (eaa). The efficiency of cyanide biodegradation from the CPME by the fe, eaa and cbe were 55, 62, and 74% respectively after 6 h. Rhodanese immobilized via cbe had a higher resistance to thermal denaturation over other enzyme forms. Hence, this makes cbe adaptable for large-scale detoxification of cyanide from CPME.

References

Jan 1, 1992·Biodegradation·S A Raybuck
Jul 5, 1992·Journal of Molecular Biology·C N Pace
Jan 1, 1981·Methods in Enzymology·J Westley
Jun 1, 1993·Journal of Biochemical Toxicology·A J NokY Sa Adatu
Mar 23, 2000·Letters in Applied Microbiology·Y B Patil, K M Paknikar
Jul 29, 2003·Biotechnology Letters·Ata Akcil, Terry Mudder
Oct 18, 2003·BMC Infectious Diseases·Priscila Gava MazzolaAlzira M da S Martins
Apr 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Andreas MatthiesSilke Leimkühler
Nov 26, 2005·Chemosphere·Rita CipollonePaolo Visca
Jan 5, 2006·Canadian Journal of Microbiology·T Kudanga, E Mwenje
Nov 14, 2006·Applied and Environmental Microbiology·Rita CipollonePaolo Visca
Mar 28, 2013·Chemical Society Reviews·Roger A Sheldon, Sander van Pelt
Sep 29, 2015·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Sujatha KandasamyGero Benckiser
Jan 1, 2013·3 Biotech·Sumitra DattaYamuna Rani Sriramulu Rajaram
Nov 21, 2017·International Journal of Biological Macromolecules·Osmar Soares da SilvaTatiana Souza Porto
May 4, 2018·International Journal of Biological Macromolecules·Rodrigo Lira de OliveiraTatiana Souza Porto
Oct 8, 2018·Bioresource Technology·Fatiha LassouaneSusana Rodriguez-Couto
Jan 17, 2019·Journal, Genetic Engineering & Biotechnology·Adedeji Nelson AdemakinwaFemi Kayode Agboola

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