PMID: 9523454Apr 2, 1998Paper

Biodegradation of cyanides, cyanates and thiocyanates to ammonia and carbon dioxide by immobilized cells of Pseudomonas putida

Journal of Industrial Microbiology & Biotechnology
K D ChapatwalaJ H Wolfram

Abstract

Pseudomonas putida utilizes cyanide as the sole source of carbon and nitrogen. Agar, alginate, and carrageenan were screened as the encapsulating matrices for P. putida. Alginate-immobilized cells of P. putida degraded sodium cyanide (NaCN) more efficiently than non-immobilized cells or cells immobilized in agar or carrageenan. The end products of biodegradation of cyanide were identified as ammonia (NH3) and carbon dioxide (CO2). These products changed the medium pH. In bioreactors, the rate of cyanide degradation increased with an increase in the rate of aeration. Maximum utilization of cyanide was observed at 200 ml min-1 of aeration. Immobilized cells of P. putida degraded cyanides, cyanates and thiocyanates to NH3 and CO2. Use of Na[14C]-CN showed that 70% of carbon of Na[14C]-CN was converted into 14CO2 and only 10% was associated with the cell biomass. The substrate-dependent kinetics indicated that the Km and Vmax values of P. putida for the substrate, NaCN were 14 mM and 29 nmol of oxygen consumed mg protein-1 min-1 respectively.

Citations

Oct 20, 2009·Applied Microbiology and Biotechnology·Ashvini U Chaudhari, Kisan M Kodam
Mar 23, 2000·Letters in Applied Microbiology·Y B Patil, K M Paknikar
Jul 29, 2008·Journal of Hazardous Materials·Rajesh Roshan DashChandrajit Balomajumder
Oct 12, 2019·Waste Management & Research : the Journal of the International Solid Wastes and Public Cleansing Association, ISWA·Cosmos AnningXianjun Lyu
Dec 10, 2015·Crisis·Kate Monaghan, Martin Harris
Jan 5, 2008·Oral Diseases·I TomásJ V Bagán
Jul 1, 2017·3 Biotech·Siraporn PotivichayanonRattana Toensakes
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May 22, 2019·Current Microbiology·Ramasamy MahendranGayathri Muthusamy
Jul 14, 2010·Organic Letters·Sukdeb SahaAmitava Das

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