Biodegradation of [D-Leu(1)] microcystin-LR by a bacterium isolated from sediment of Patos Lagoon estuary, Brazil

The Journal of Venomous Animals and Toxins Including Tropical Diseases
Gilmar A F LemesJoão Sarkis Yunes

Abstract

Toxic cyanobacterial blooms are recurrent in Patos Lagoon, in southern Brazil. Among cyanotoxins, [D-Leu(1)] microcystin-LR is the predominant variant whose natural cycle involves water and sediment compartments. This study aimed to identify and isolate from sediment a bacterial strain capable of growing on [D-Leu(1)] microcystin-LR. Sediment and water samples were collected at two distinct aquatic spots: close to the Oceanographic Museum (P1), in Rio Grande City, and on São Lourenço Beach (P2), in São Lourenço do Sul City, southern Brazil. [D-Leu(1)] microcystin-LR was isolated and purified from batch cultures of Microcystis aeruginosa strain RST9501. Samples of water and sediment from Rio Grande and São Lourenço do Sul were collected. Bacteria from the samples were allowed to grow in flasks containing solely [D-Leu(1)] microcystin-LR. This strain named DMSX was isolated on agar MSM with 8 g L(-1) glucose and further purified on a cyanotoxin basis growth. Microcystin concentration was obtained by using the ELISA immunoassay for microcystins whereas bacterial count was performed by epifluorescence microscopy. The genus Pseudomonas was identified by DNA techniques. Although several bacterial strains were isolated from the sample...Continue Reading

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Citations

Dec 5, 2015·Frontiers in Microbiology·Rajesh P RastogiAran Incharoensakdi
Mar 25, 2015·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Daniela Fernandes RamosJoão Sarkis Yunes
Feb 2, 2019·Bioinspiration & Biomimetics·Yuki AsanoMasayuki Inaba
Oct 31, 2018·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Isaac Yaw MasseyFei Yang
Apr 25, 2020·Toxins·Isaac Yaw Massey, Fei Yang
May 20, 2021·Environmental Science and Pollution Research International·Gabriela Rocío Sosa BenegasMichel Rodrigo Zambrano Passarini

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Datasets Mentioned

BETA
YL84
HQ890467

Methods Mentioned

BETA
ELISA
PCR

Software Mentioned

Basic Local Alignment Search Tool ( BLAST
MEGA
UTHSCSA Image Tool
ClustalX

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