Biodegradation of keratinous waste by Chryseobacterium sp. RBT isolated from soil contaminated with poultry waste

Journal of Basic Microbiology
Ranjit G Gurav, Jyoti P Jadhav

Abstract

In the present study, a feather degrading bacterial strain was isolated from poultry waste disposal site, Kolhapur, India. The bacterium was identified as Chryseobacterium sp. RBT using 16S rRNA gene sequence analysis. Chryseobacterium sp. RBT showed rapid hydrolysis of native feathers within 30 h and produced the highest level of keratinase activity (98.3 U/ml). Keratin containing wastes viz. silk, human hair, wool and chicken feathers were tested for keratin degrading ability of the bacterium. Amongst the tested substrates, the Chryseobacterium sp. RBT showed more specificity towards chicken feathers (98.6% degradation) with maximum keratinase activity (98.3 U/ml) and solubilized protein concentration (3.84 mg/ml). Effect of various physico-chemical parameters (temperature, pH, carbon and nitrogen sources) on keratinase production was monitored. The maximum keratinase activity was observed at pH (8.6) and temperature (50 °C). Molasses (1.0% w/v) acted as an inducer and enhanced the keratinolytic activity by two fold, while starch worked as an inhibitor. The goat skin when treated with crude keratinase enzyme (2% v/v), showed complete dehairing within 12 h. Hence, Chryseobacterium sp. RBT shows potential as a candidate for tre...Continue Reading

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Citations

Sep 10, 2013·Applied Biochemistry and Biotechnology·I DarahSheh-Hong Lim
Feb 19, 2013·BMC Biotechnology·Ana Lúcia Vazquez VillaAlane Beatriz Vermelho
Apr 22, 2016·Microbes and Environments·Tomoki NishiokaMasafumi Shimizu
Sep 19, 2017·Applied Biochemistry and Biotechnology·Priya FukeAnshuman A Khardenavis
Apr 1, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nonso E NnolimUchechukwu U Nwodo
Oct 7, 2018·General and Comparative Endocrinology·Andrew C AlbaDylan C Kesler
Apr 28, 2021·Current Microbiology·Ranjeeta BhariRam Sarup Singh
Jun 1, 2021·Environmental Science and Pollution Research International·Charlene S AnjosAndré L M Porto

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