Biodegradation of phthalic acid esters (PAEs) and in silico structural characterization of mono-2-ethylhexyl phthalate (MEHP) hydrolase on the basis of close structural homolog

Journal of Hazardous Materials
Neha SinghPravindra Kumar

Abstract

Three bacterial strains capable of degrading phthalates namely Pseudomonas sp. PKDM2, Pseudomonas sp. PKDE1 and Pseudomonas sp. PKDE2 were isolated and characterized for their degradative potential. These strains efficiently degraded 77.4%-84.4% of DMP, 75.0%-75.7% of DEP and 71.7%-74.7% of DEHP, initial amount of each phthalate is 500mgL-1 of each phthalate, after 44h of incubation. GC-MS results reveal the tentative DEHP degradation pathway, where hydrolases mediate the breakdown of DEHP to phthalic acid (PA) via an intermediate MEHP. MEHP hydrolase is a serine hydrolase which is involved in the reduction of the MEHP to PA. The predicted 3D model of MEHP hydrolase from Pseudomonas mosselii was docked with phthalate monoesters (PMEs) such as MEHP, mono-n-hexyl phthalate (MHP), mono-n-butyl phthalate (MBP) and mono-n-ethyl phthalate (MEP), respectively. Docking results show the distance between the carbonyl carbon of respective phthalate monoester and the hydroxyl group of catalytic serine lies in the range of 2.9 to 3.3Å, which is similar to the ES complex of other serine hydrolases. This structural study highlights the interaction and the role of catalytic residues of MEHP hydrolase involved in the biodegradation of PMEs to p...Continue Reading

Citations

Oct 27, 2020·Journal of Biomolecular Structure & Dynamics·Neema KumariSubha Narayan Rath
Dec 21, 2020·Ecotoxicology and Environmental Safety·Jiarong QiuZhihong Xin
Jan 24, 2021·Journal of Hazardous Materials·Ericka Santacruz-JuárezCarmen Sánchez
Sep 25, 2020·Archives of Biochemistry and Biophysics·Poonam DhankharPravindra Kumar
May 4, 2021·Journal of Biomolecular Structure & Dynamics·Deena Nath GuptaAshwani Kumar Sharma

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