Biodegradation of pyraclostrobin by two microbial communities from Hawaiian soils and metabolic mechanism

Journal of Hazardous Materials
Xiaoxin ChenXiaolu Liu

Abstract

Pyraclostrobin has been widely and long-termly applicated to agricultural fields. The removal of pyraclostrobin from ecological environment has received wide attention. In this study, using sequential enrichments with pyraclostrobin as a sole carbon source, two microbial communities (HI2 and HI6) capable of catabolizing pyraclostrobin were obtained from Hawaiian soils. The microfloras analysis indicated that only Proteobacteria and Bacteroides could survive in HI2-soil after acclimatization, whereas the number of Proteobacteria in HI6-soil accounted for more than 99%. The percentages of Pseudomonas in the HI2 and HI6 microfloras were 69.3% and 59.3%, respectively. More than 99% of pyraclostrobin (C0 = 100 mg L-1) was degraded by the HI2 and HI6 microorganisms within five days. A unique metabolite was identified by high performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry (HPLC-QTOF-MS/MS). A metabolic pathway involving carbamate hydrolysis was proposed. The tertiary amine group of pyraclostrobin was hydrolyzed to primary amine group with the decarboxylation, which facilitated pyraclostrobin detoxification because carboxylester was an important functional group. The metabolic mechanism suggested t...Continue Reading

Citations

Apr 1, 2020·Frontiers in Microbiology·Yanmei FengShaohua Chen
Mar 19, 2021·Critical Reviews in Biotechnology·Pankaj BhattShaohua Chen
Mar 21, 2021·Applied and Environmental Microbiology·Wankui JiangQing Hong
Jun 5, 2021·Colloids and Surfaces. B, Biointerfaces·Ummie Umaiera Mohd JohanMohd Shukuri Mohamad Ali
Jul 27, 2021·Frontiers in Microbiology·Harshit MalhotraPrashant S Phale
Jan 4, 2022·The Science of the Total Environment·Weijun WangXiaolu Liu

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