Biodegradation of [(sup14)C]Benzo[a]pyrene Added in Crude Oil to Uncontaminated Soil.

Applied and Environmental Microbiology
R A KanalyM Findlay

Abstract

To investigate the possible cometabolic biodegradation of benzo[a]pyrene (BaP), crude oil spiked with [7-(sup14)C]BaP and unlabeled BaP was added to soil with no known pollution history, to give 34 g of oil and 67 mg of BaP/kg of dry soil. The oil-soil mixture was amended with mineral nutrients and incubated in an airtight container with continuous forced aeration. Total CO(inf2) and (sup14)CO(inf2) in the off-gas were trapped and quantified. Soil samples were Soxhlet extracted with dichloromethane at seven time points during the 150-day incubation period, and the extracted soil was subjected to further fractionation in order to recover reversibly and irreversibly bound radiocarbon. Radiocarbon recovery was 100% (plusmn) 3% for each time point. During the first 50 days of incubation, no (sup14)CO(inf2) was evolved, but over the next 100 days, 50% of the BaP radiocarbon was evolved as (sup14)CO(inf2). At 150 days, only 5% of the intact BaP and 23% of the crude oil remained. Of the remaining radiolabel, 20% was found in solvent-extractable metabolites and 25% was incorporated into soil organic matter. Only 1/10 of this could be solubilized by chemical hydrolysis. An abiotic control experiment exhibited binding of only 2% of the B...Continue Reading

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Citations

Sep 30, 2000·Applied and Environmental Microbiology·R A KanalyS Harayama
Jan 25, 2011·Microbial Biotechnology·Robert A Kanaly, Shigeaki Harayama
Mar 29, 2000·Journal of Bacteriology·R A Kanaly, S Harayama
Dec 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Jonathan D Van HammeOwen P Ward
Nov 27, 2002·Applied and Environmental Microbiology·Robert A KanalyKazuya Watanabe
Aug 26, 2015·MicrobiologyOpen·Robert A KanalyNatsuko Hamamura
May 28, 2015·International Journal of Systematic and Evolutionary Microbiology·Allyn H MaedaRobert A Kanaly
Oct 1, 1999·Environmental Toxicology and Chemistry·Robert A Kanaly, Richard Bartha
Dec 17, 2008·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Mathava KumarJih-Gaw Lin
May 5, 2009·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Shaw Y YuanBea V Chang
May 1, 1999·Applied and Environmental Microbiology·M KästnerW Fritsche
Jan 6, 2021·International Journal of Environmental Research and Public Health·Alexis Nzila, Musa M Musa
Nov 26, 2003·FEMS Microbiology Letters·Philip H LeeKirk T Semple
May 14, 2020·Chemosphere·Ruihuan ZhangQuankai Fu
May 21, 2013·Applied and Environmental Microbiology·Marie KunihiroRobert A Kanaly
Oct 6, 2007·Journal of Environmental Sciences (China)·Shu-yan ZangJing Chen

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