Biodegradation of vinyl chloride and cis-dichloroethene by a Ralstonia sp. strain TRW-1

Applied Microbiology and Biotechnology
Vijai K ElangoBabu Z Fathepure

Abstract

An aerobic bacterium, Ralstonia sp. strain TRW-1, that assimilates vinyl chloride (VC) or ethene (ETH) as the sole carbon source was isolated from a chloroethene-degrading enrichment culture. Phylogenetic analysis of 16S rDNA sequence of the isolate revealed almost 99% sequence similarity to Ralstonia pickettii. To our knowledge, this is the first report describing the isolation of a member of Ralstonia that can degrade VC as the growth substrate. The measured growth yield values for VC and ETH were 11.27 and 18.90 g protein/mole, respectively. The estimated half-velocity constant K (m) values for VC and ETH were 9.09+/-2.97 and 5.73+/-2.96 muM, respectively. These values are almost three- to tenfold higher than for other VC-assimilating Mycobacterium sp. The strain also degrades cis-dichloroethene (cis-DCE) in mineral salts medium containing yeast-extract, beef-extract, casamino acids, or peptone. This ability of the strain TRW-1 to degrade cis-DCE in the presence of a nontoxic, water-soluble substrate is relevant to in-situ remediation of cis-DCE-contaminated aquifers.

References

Apr 1, 1992·Applied and Environmental Microbiology·S Hartmans, J A De Bont
Jun 1, 1994·Journal of Bacteriology·R H OlsenB Kaphammer
Jul 2, 1998·Applied and Environmental Microbiology·P SteinleK W Hanselmann
Jul 25, 2000·Environmental Health Perspectives·J KielhornI Mangelsdorf
Aug 5, 2000·Applied and Environmental Microbiology·M F VerceD L Freedman
Nov 17, 2001·Biodegradation·L Alvarez-Cohen, G E Speitel
Jun 1, 2002·Environmental Science & Technology·Matthew F VerceDavid L Freedman
Jun 1, 2002·Applied and Environmental Microbiology·Nicholas V ColemanJim C Spain
Nov 27, 2002·Applied and Environmental Microbiology·Nicholas V ColemanJim C Spain
Jul 2, 2004·Biodegradation·Harvinder SinghBabu Z Fathepure
Sep 24, 2004·Environmental Science & Technology·Benjamin M GriffinFrank E Löffler
Oct 7, 2004·Applied and Environmental Microbiology·Anthony S DankoDavid L Freedman
Dec 14, 2005·Applied and Environmental Microbiology·P M BradleyR S Dinicola
Oct 1, 1996·Applied and Environmental Microbiology·F E LofflerJ M Tiedje
Mar 15, 2006·Applied and Environmental Microbiology·V J Van HylckamaD B Janssen

❮ Previous
Next ❯

Citations

May 10, 2011·Journal of Bacteriology·Nicholas V ColemanTimothy E Mattes
May 20, 2015·Applied Microbiology and Biotechnology·Fernanda PaesAlison M Cupples
Feb 12, 2010·FEMS Microbiology Reviews·Timothy E MattesNicholas V Coleman
Jun 28, 2016·Environmental Science and Pollution Research International·Fernanda Paes WilsonAlison M Cupples
Jan 31, 2012·Applied and Environmental Microbiology·Christine A Rabinovitch-Deere, Rebecca E Parales
Jan 4, 2018·Applied Microbiology and Biotechnology·Anne E TaylorLewis Semprini
Apr 6, 2017·Environmental Science and Pollution Research International·Iva DolinováAlena Ševců
Jan 26, 2021·Journal of Microbiological Methods·Patrick M Richards, Timothy E Mattes
Jul 21, 2021·Journal of Hazardous Materials·Luc MalhautierJean-Louis Fanlo
Oct 24, 2007·Environmental Science & Technology·Anne E TaylorLewis Semprini

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioremediation (ASM)

Bioremediation is the treatment and removal of harmful pollutants or contaminants through the use of microorganisms. Discover the latest research here.