Abstract
Methyl ethyl ketone (MEK) and methyl iso-butyl ketone (MIBK) constitute significant proportion of the total VOC emissions from manufacturing and application processes of surface coatings. Biodegradation of MEK and MIBK using an acclimatized mixed culture was evaluated, under aerobic condition. Biodegradation studies were carried out using MEK and MIBK as single substrates and in combination. Mixed-pollutant studies were conducted in MEK-dominated system, MIBK-dominated system, and MEK-MIBK equi-concentration systems to understand the concentration-dependent interaction of these compounds in a biosystem. Experimental data obtained from single-pollutant system was used to estimate the biokinetic parameters, viz. μ(max), K(s), K(i), and Y(T), for these compounds. Among the several bio-kinetic models tested, Monod inhibition model was best suited for predicting the biodegradation of these two VOCs. Four multiple-substrate models, viz. no-interaction, competitive, un-competitive, and non-competitive were used to study the nature of inhibition for different combinations of these compounds. The biodegradation of MEK and MIBK mixtures was found to be best described by competitive inhibition model. However, the predictions were not very...Continue Reading
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