Biodistribution and catabolism of 18F-labelled isopeptide N(epsilon)-(gamma-glutamyl)-L-lysine

Amino Acids
C HultschThomas Henle


Isopeptide bonds between the epsilon-amino group of lysine and the gamma-carboxamide group of glutamine are formed during strong heating of pure proteins or, more important, by enzymatic reaction mediated by transglutaminases. Despite the wide use of a microbial transglutaminase in food biotechnology, up to now little is known about the metabolic fate of the isopeptide N(epsilon)-(gamma-glutamyl)-L-lysine. In the present study, N-succinimidyl-4-[(18)F]fluorobenzoate was used to modify N(epsilon)-(gamma-glutamyl)-L-lysine at each of its two alpha-amino groups, resulting in the 4-[(18)F]fluorobenzoylated derivatives, for which biodistribution, catabolism, and elimination were investigated in male Wistar rats. A significant different biochemical behavior of the two labelled isopeptides was observed in terms of in vitro stability, in vivo metabolism as well as biodistribution. The results suggest that the metabolic fate of isopeptides is likely to be dependent on how they are reabsorbed - free or peptide bound.


Jun 1, 1975·Canadian Journal of Biochemistry·A Szewczuk, G E Connell
Jan 1, 1978·Advances in Experimental Medicine and Biology·P A FinotJ Mauron
Sep 1, 1975·The British Journal of Nutrition·G RaczyńskiS Buraczewski
Jan 1, 1990·Journal of Nutritional Science and Vitaminology·K Yasumoto, F Suzuki
Jan 1, 1985·Methods in Enzymology·S S Tate, A Meister
May 1, 1972·The British Journal of Nutrition·P E Waibel, K J Carpenter
Apr 11, 1997·The Journal of Biological Chemistry·K N ParameswaranL Lorand
Oct 9, 2002·The Biochemical Journal·Martin GriffinCarlo M Bergamini
Jul 25, 2003·Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine·F WüstT Henle

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