Biodiversity of Environmental Leptospira : Improving Identification and Revisiting the Diagnosis

Frontiers in Microbiology
Roman ThibeauxCyrille Goarant

Abstract

Leptospirosis is an important environmental disease and a major threat to human health causing at least 1 million clinical infections annually. There has recently been a growing interest in understanding the environmental lifestyle of Leptospira. However, Leptospira isolation from complex environmental samples is difficult and time-consuming and few tools are available to identify Leptospira isolates at the species level. Here, we propose a polyphasic isolation and identification scheme, which might prove useful to recover and identify environmental isolates and select those to be submitted to whole-genome sequencing. Using this approach, we recently described 12 novel Leptospira species for which we propose names. We also show that MALDI-ToF MS allows rapid and reliable identification and provide an extensive database of Leptospira MALDI-ToF mass spectra, which will be valuable to researchers in the leptospirosis community for species identification. Lastly, we also re-evaluate some of the current techniques for the molecular diagnosis of leptospirosis taking into account the extensive and recently revealed biodiversity of Leptospira in the environment. In conclusion, we describe our method for isolating Leptospira from the en...Continue Reading

References

Sep 1, 1992·Journal of Clinical Microbiology·F MérienI Saint Girons
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Sep 1, 1986·Journal of Clinical Microbiology·G P SchmidD J Brenner
May 1, 1970·The American Journal of Tropical Medicine and Hygiene·M F Baker, H J Baker
Sep 12, 1998·International Journal of Systematic Bacteriology·P PerolatM S Serrano
Jul 9, 2002·BMC Infectious Diseases·Lee D SmytheDavid B McKay
Oct 1, 1961·Canadian Journal of Microbiology·J G COUSINEAU, J A McKIEL
Jul 12, 2005·FEMS Microbiology Letters·Fabrice MerienGuy Baranton
Mar 4, 2006·International Journal of Systematic and Evolutionary Microbiology·Paul N LevettArnold G Steigerwalt
Nov 24, 2006·Annals of Clinical Microbiology and Antimicrobials·Niyaz AhmedRudy A Hartskeerl
Oct 10, 2008·International Journal of Systematic and Evolutionary Microbiology·Andrew T SlackLee D Smythe
Mar 31, 2009·International Journal of Systematic and Evolutionary Microbiology·Andrew T SlackLee D Smythe
Apr 28, 2009·Diagnostic Microbiology and Infectious Disease·Robyn A StoddardAlex R Hoffmaster
May 7, 2011·Microbiology and Immunology·Antara ChakrabortyShin-Ichi Yoshida
Nov 4, 2011·Journal of Water and Health·Emily J Viau, Alexandria B Boehm
Mar 6, 2012·Veterinary Microbiology·Zoheira DjelouadjiMichel Drancourt
Nov 13, 2012·Applied and Environmental Microbiology·Mitsumasa SaitoShin-ichi Yoshida
Oct 8, 2013·PLoS Neglected Tropical Diseases·Jason S LehmannMichael A Matthias
Jun 4, 2014·BMC Research Notes·Adriana CalderaroMaria Cristina Medici
Sep 25, 2014·International Journal of Systematic and Evolutionary Microbiology·Pascale BourhyMathieu Picardeau
Dec 2, 2014·Pathogens·Jason S LehmannDerrick E Fouts
Sep 18, 2015·PLoS Neglected Tropical Diseases·Federico CostaAlbert I Ko
Feb 20, 2016·PLoS Neglected Tropical Diseases·Derrick E FoutsJoseph M Vinetz
Feb 13, 2017·Diagnostic Microbiology and Infectious Disease·Dominique GiraultCyrille Goarant
Feb 22, 2017·Emerging Infectious Diseases·Motoyuki TsuboiYasuyuki Kato
Feb 28, 2017·PLoS Neglected Tropical Diseases·Roman ThibeauxCyrille Goarant
Mar 7, 2017·Nature Reviews. Microbiology·Mathieu Picardeau
May 14, 2017·The American Journal of Tropical Medicine and Hygiene·Ronald G Peláez SánchezPiedad Agudelo-Flórez
Jul 19, 2017·Parasitology·Vanina GuernierCyrille Goarant
Nov 3, 2017·Japanese Journal of Infectious Diseases·Somjit ChaiwattanarungruengpaisanMetawee Thongdee
Dec 15, 2017·International Journal of Systematic and Evolutionary Microbiology·Rafael PucheGregorio Iraola

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Citations

Sep 15, 2018·International Journal of Environmental Health Research·Kevin Escandón-VargasMiryam Astudillo-Hernández
Oct 10, 2018·The American Journal of Tropical Medicine and Hygiene·Arnaud Tarantola, Cyrille Goarant
Feb 1, 2019·Transboundary and Emerging Diseases·Anahi S VieiraWalter Lilenbaum
Apr 27, 2019·PLoS Neglected Tropical Diseases·Julien GuglielminiMathieu Picardeau
May 24, 2019·PLoS Neglected Tropical Diseases·Antony T VincentMathieu Picardeau
Jun 12, 2019·International Journal of Systematic and Evolutionary Microbiology·Arnau Casanovas-MassanaElsio A Wunder
Aug 25, 2019·Microbiology and Immunology·Toshiyuki MasuzawaShuichi Nakamura
Jun 4, 2019·Japanese Journal of Infectious Diseases·Metawee ThongdeeWitthawat Wiriyarat
Jan 28, 2020·PloS One·Emilie BierqueCyrille Goarant
May 21, 2019·PLoS Neglected Tropical Diseases·Kathryn M BenavidezIván Castro-Arellano
Nov 21, 2019·Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz·Lisa H NauAnne Mayer-Scholl
Nov 23, 2019·Microbiology Resource Announcements·Cecilia NievesGregorio Iraola
Oct 1, 2020·International Journal of Systematic and Evolutionary Microbiology·Aharon Oren, George M Garrity
Sep 28, 2018·Frontiers in Cellular and Infection Microbiology·Elsio A WunderNadia Benaroudj
Mar 19, 2020·Revista Da Sociedade Brasileira De Medicina Tropical·Rafael Guillermo Villarreal JulioRonald Guillermo Peláez Sánchez
Jan 21, 2021·Animals : an Open Access Journal From MDPI·Giovanni CiliaFilippo Fratini
Feb 9, 2021·Tropical Animal Health and Production·Maria Catalina Ospina-Pinto, Patricia Hernández-Rodríguez
Dec 10, 2020·Tropical Medicine and Infectious Disease·Somjit ChaiwattanarungruengpaisanMetawee Thongdee
Jan 26, 2021·Comparative Immunology, Microbiology and Infectious Diseases·Mara L MartinezBibiana F Brihuega
Feb 23, 2021·Epidemiology and Infection·K JeskeC Imholt
May 1, 2021·Animals : an Open Access Journal From MDPI·Ivana PireddaValentina Chisu
Jul 6, 2021·Comparative Immunology, Microbiology and Infectious Diseases·Vanina SaraulloBibiana Brihuega
Aug 24, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Sérgio Ricardo MaiolinoMárcio Garcia Ribeiro
Jan 5, 2022·Current Microbiology·Camila L CamposJosefa B Da Silva

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Datasets Mentioned

BETA
NPDS00000000
NPDX00000000

Methods Mentioned

BETA
Genome Sequencing
PCR
genotyping
scanning electron microscopy

Software Mentioned

LightCycler 480
CLC Genomics Workbench
flexControl
MALDI Biotyper

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