Biodynamic digital holography of chemoresistance in a pre-clinical trial of canine B-cell lymphoma

Biomedical Optics Express
Honggu ChoiDavid Nolte

Abstract

Biodynamic digital holography was used to obtain phenotypic profiles of canine non-Hodgkin B-cell lymphoma biopsies treated with standard-of-care chemotherapy. Biodynamic signatures from the living 3D tissues were extracted using fluctuation spectroscopy from intracellular Doppler light scattering in response to the molecular mechanisms of action of therapeutic drugs that modify a range of internal cellular motions. The standard-of-care to treat B-cell lymphoma in both humans and dogs is a combination CHOP therapy that consists of doxorubicin, prednisolone, cyclophosphamide and vincristine. The proportion of dogs experiencing durable cancer remission following CHOP chemotherapy was 68%, with 13 out of 19 dogs responding favorably to therapy and 6 dogs failing to have progression-free survival times greater than 100 days. Biodynamic signatures were found that correlate with inferior survival times, and biomarker selection was optimized to identify specific Doppler signatures related to chemoresistance. A machine learning classifier was constructed based on feature vector correlations and linear separability in high-dimensional feature space. Hold-out validation predicted patient response to therapy with 84% accuracy. These resul...Continue Reading

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Citations

Dec 18, 2019·BMC Veterinary Research·Amadeo M ParissentiBrenda L Coomber
May 3, 2019·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Zhe LiDavid D Nolte
Jun 27, 2019·Journal of Biomedical Optics·Zhe LiDavid Nolte
Feb 12, 2021·Communications Biology·Honggu ChoiDavid D Nolte
Apr 23, 2021·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Alessandro FerrariValeria Martini

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