Abstract
The aim of this study was to study the bioeffects of diagnostic dynamic 3-dimensional ultrasound (4D) on ultrastructure of cerebral cells of fetal mice in late pregnancy. Thirty pregnant mice carrying 18th embryonic day fetuses were randomly allocated into 6 groups, namely, control group, sham-exposed group, 5 minute-exposed group, 10 minute-exposed group, 20 minute-exposed group, and 30 minute-exposed groups (5 in each group). In exposure groups, mice were put under the dynamic 3D ultrasound system's probe for 5 to 30 minutes. Mice in sham-exposed group did not receive ultrasound wave. At 24th hour after birth, 10 pups of each group were randomly selected (2 in each litter) and euthanized by decapitation, and the brains were immediately removed. Right parietal lobes were taken as specimen. The specimens were firstly fixed with glutaraldehyde and secondly with osmic acid, then sections were made and observed under the transmission electron microscope. There were no obvious abnormal ultrastructure changes in control group, sham-exposed group, and 5 minute-exposed group under transmission electron microscope. Ten minute-exposed group showed some enlarged mitochondria, broken crista, vacuolated endoplasmic resticulums, and a few a...Continue Reading
References
Jan 1, 1990·Ultrasound in Medicine & Biology·S Z ChildE L Carstensen
Jan 1, 1987·Ultrasonics·C L ChristmanP Riesz
Jan 1, 1995·Ultrasound in Medicine & Biology·D DaleckiE L Carstensen
May 5, 1999·Lancet·K TachibanaK Tamura
Jul 1, 1999·Ultrasound in Medicine & Biology·M M HorderA K Wood
Nov 3, 2005·Birth Defects Research. Part A, Clinical and Molecular Teratology·John M Graham
Aug 12, 2006·Proceedings of the National Academy of Sciences of the United States of America·Eugenius S B C AngPasko Rakic
Sep 5, 2006·Progress in Biophysics and Molecular Biology·Charles C Church, Morton W Miller
Feb 1, 2007·Ultrasound in Obstetrics & Gynecology : the Official Journal of the International Society of Ultrasound in Obstetrics and Gynecology·E SheinerJ S Abramowicz
Feb 14, 2008·Ultraschall in der Medizin : Organ der Deutschen Gesellschaft für Ultraschall in der Medizin, [der] Österreichischen Gesellschaft für Ultraschall in der Medizin, [der] Schweizerischen Gesellschaft für Ultraschall in Medizin und Biologie·M ClaudonT Whittingham
Mar 25, 2008·Journal of Ultrasound in Medicine : Official Journal of the American Institute of Ultrasound in Medicine·Melvin E StratmeyerKjell A Salvesen
Jul 19, 2008·Medical Engineering & Physics·Francis A Duck
Aug 10, 2010·Ultrasound in Medicine & Biology·Jacques S Abramowicz