Bioelectrical impedimetric sensor for single cell analysis based on nanoroughened quartz substrate; suitable for cancer therapeutic purposes

Journal of Pharmaceutical and Biomedical Analysis
Milad Gharooni, Mohammad Abdolahad

Abstract

Single cells analysis has been interested in recent decade. Apart from scientific benefits to achieve new biological phenomena in cell study, many diagnostic and therapeutic protocols in non-communicable diseases were introduced by single cell analysis. Moreover, non-invasive methods to maintain the investigated cell for time dependent monitoring has been widely studied because of its importance in some crucial cases such as drug resistance in cancer. Bioelectrical monitoring is one of such methods Although the procedures reported based on electrical probing might not induce cell disruption, indirect connection between recording electrodes and cell membrane (mostly in microfluidic approaches) reduced the quality of response and limited the precision of the results. Here, a bioelectronic sensor for monitoring the effect of anticancer drugs on single breast cancer cells was fabricated based on nano-roughened gold electrodes on a quartz substrate applied direct contacts to cell membrane. Whole of the surface except a microcircle surrounded the sensing region was passivated by overbaked photoresist layer. Cells were dropped on the sensor without the assistance of any micropipette or microfluidic systems and just individual regions ...Continue Reading

Citations

Sep 4, 2020·Micromachines·Lexi L CrowellTayloria N G Adams
Jun 27, 2019·Biosensors & Bioelectronics·Niharika GuptaBansi D Malhotra

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