Bioenergetic Response of the Extreme Thermoacidophile Metallosphaera sedula to Thermal and Nutritional Stresses.

Applied and Environmental Microbiology
T L Peeples, Robert M Kelly

Abstract

The bioenergetic response of the extremely thermoacidophilic archaeon Metallosphaera sedula to thermal and nutritional stresses was examined. Continuous cultures (pH 2.0, 70(deg)C, and dilution rate of 0.05 h(sup-1)) in which the levels of Casamino Acids and ferrous iron in growth media were reduced by a step change of 25 to 50% resulted in higher levels of several proteins, including a 62-kDa protein immunologically related to the molecular chaperone designated thermophilic factor 55 in Sulfolobus shibatae (J. D. Trent, J. Osipiuk, and T. Pinkau, J. Bacteriol. 172:1478-1484, 1990), on sodium dodecyl sulfate-polyacrylamide gels. The 62-kDa protein was also noted at elevated levels in cells that had been shifted from 70 to either 80 or 85(deg)C. The proton motive force ((Delta)p), transmembrane pH ((Delta)pH), and membrane potential ((Delta)(psi)) were determined for samples obtained from continuous cultures (pH 2.0, 70(deg)C, and dilution rate of 0.05 h(sup-1)) and incubated under nutritionally and/or thermally stressed and unstressed conditions. At 70(deg)C under optimal growth conditions, M. sedula was typically found to have a (Delta)p of approximately -190 to -200 mV, the result of an intracellular pH of 5.4 (extracellular ...Continue Reading

References

Oct 13, 1992·Annals of the New York Academy of Sciences·A Matin
Jan 11, 1980·Nucleic Acids Research·B BowenH Weintraub
Dec 1, 1983·Microbiological Reviews·J G Cobley, J C Cox
Aug 1, 1993·Applied and Environmental Microbiology·T R ClarkG J Olson
Feb 2, 1973·Science·C L Brierley, L E Murr

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Citations

Jul 1, 1995·Bio/technology·M W AdamsR M Kelly
Jan 16, 2009·Biochemical Society Transactions·Charlotte R CooperRobert M Kelly
Dec 16, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jonathan D TrentChristopher E Henze
Jan 22, 2021·FEMS Microbiology Reviews·April M LewisRobert M Kelly
Dec 10, 1999·Microbiology and Molecular Biology Reviews : MMBR·A J MacarioE Conway de Macario

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