Bioengineering triacetic acid lactone production in Yarrowia lipolytica for pogostone synthesis

Biotechnology and Bioengineering
James YuSuzanne Sandmeyer

Abstract

Yarrowia lipolytica is an oleaginous yeast that is recognized for its ability to accumulate high levels of lipids, which can serve as precursors to biobased fuels and chemicals. Polyketides, such as triacetic acid lactone (TAL), can also serve as a precursor for diverse commodity chemicals. This study used Y. lipolytica as a host organism for the production of TAL via expression of the 2-pyrone synthase gene from Gerbera hybrida. Induction of lipid biosynthesis by nitrogen-limited growth conditions increased TAL titers. We also manipulated basal levels of TAL production using a DNA cut-and-paste transposon to mobilize and integrate multiple copies of the 2-pyrone synthase gene. Strain modifications and batch fermentation in nitrogen-limited medium yielded TAL titers of 2.6 g/L. Furthermore, we show that minimal medium allows TAL to be readily concentrated at >94% purity and converted at 96% yield to pogostone, a valuable antibiotic. Modifications of this reaction scheme yielded diverse related compounds. Thus, oleaginous organisms have the potential to be flexible microbial biofactories capable of economical synthesis of platform chemicals and the generation of industrially relevant molecules.

References

Mar 15, 2003·Natural Product Reports·Michael B Austin, Joseph P Noel
Nov 24, 2004·Molecular Microbiology·Lori A Maggio-Hall, Nancy P Keller
Sep 2, 2009·Progress in Lipid Research·Athanasios BeopoulosJean-Marc Nicaud
Jun 1, 2012·Journal of Industrial Microbiology & Biotechnology·Ludwika TomaszewskaWitold Gładkowski
Aug 2, 2014·Metabolic Engineering·Javier Cardenas, Nancy A Da Silva
Feb 16, 2015·Journal of Industrial Microbiology & Biotechnology·Lauren P SaundersRonald E Hector
Mar 4, 2015·Metabolic Engineering·Kangjian QiaoGregory Stephanopoulos
May 20, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mallappa Kumara Swamy, Uma Rani Sinniah
Jun 1, 2016·Journal of Asian Natural Products Research·Han-Bin ChenHui-Fang Zeng
Mar 16, 2017·Biotechnology and Bioengineering·Peng XuGregory Stephanopoulos
Aug 3, 2017·Bioresource Technology·Weizhu ZengJingwen Zhou
Feb 15, 2018·Proceedings of the National Academy of Sciences of the United States of America·Kelly A MarkhamHal S Alper

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Citations

Mar 11, 2020·Annual Review of Chemical and Biomolecular Engineering·Jiajie Huo, Brent H Shanks
Nov 7, 2019·Applied Microbiology and Biotechnology·Kelly K Miller, Hal S Alper
Apr 16, 2020·Biotechnology and Applied Biochemistry·Rajesh K SrivastavaSarat Babu Imandi
Jan 1, 2020·Biotechnology for Biofuels·Yinghang LiuJin Hou
Jul 27, 2018·Journal of Industrial Microbiology & Biotechnology·Ye LiPatrick C Cirino
Mar 25, 2019·Biotechnology and Bioengineering·Tami L McTaggartNancy A Da Silva
Feb 23, 2021·Frontiers in Bioengineering and Biotechnology·Jian ZhaXia Wu
Jul 30, 2018·Metabolic Engineering·Ahmad M Abdel-MawgoudHal S Alper
May 19, 2021·Critical Reviews in Biotechnology·Patrycja SzczepańskaZbigniew Lazar

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