Bioethanol production from the nutrient stress-induced microalga Chlorella vulgaris by enzymatic hydrolysis and immobilized yeast fermentation

Bioresource Technology
Kyoung Hyoun KimHyeun-Jong Bae

Abstract

The microalga Chlorella vulgaris is a potential feedstock for bioenergy due to its rapid growth, carbon dioxide fixation efficiency, and high accumulation of lipids and carbohydrates. In particular, the carbohydrates in microalgae make them a candidate for bioethanol feedstock. In this study, nutrient stress cultivation was employed to enhance the carbohydrate content of C. vulgaris. Nitrogen limitation increased the carbohydrate content to 22.4% from the normal content of 16.0% on dry weight basis. In addition, several pretreatment methods and enzymes were investigated to increase saccharification yields. Bead-beating pretreatment increased hydrolysis by 25% compared with the processes lacking pretreatment. In the enzymatic hydrolysis process, the pectinase enzyme group was superior for releasing fermentable sugars from carbohydrates in microalgae. In particular, pectinase from Aspergillus aculeatus displayed a 79% saccharification yield after 72h at 50°C. Using continuous immobilized yeast fermentation, microalgal hydrolysate was converted into ethanol at a yield of 89%.

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Citations

Sep 17, 2014·Bioresource Technology·Ahmed MahdyCristina González-Fernández
Dec 3, 2014·Bioresource Technology·Ho Myeong KimHyeun-Jong Bae
Aug 26, 2015·Biotechnology Advances·Marie DemuezMercedes Ballesteros
Oct 29, 2015·Bioprocess and Biosystems Engineering·Marwa M EldalatonyByong-Hun Jeon
Oct 12, 2015·Bioresource Technology·Cristina Gonzalez-FernandezBeatriz Molinuevo-Salces
Mar 3, 2015·Biotechnology Advances·Chung Hong TanJohn Chi-Wei Lan
May 16, 2015·Biotechnology and Bioengineering·Marie DemuezMercedes Ballesteros
May 16, 2015·Bioresource Technology·Sun-A ChoiJi-Yeon Park
Oct 21, 2016·Microbial Biotechnology·Forough Ghasemi NaghdiPeer M Schenk
Mar 28, 2017·Marine Drugs·Antonio Trincone
Jun 28, 2018·Macromolecular Rapid Communications·Bin HeWantai Yang
Oct 10, 2018·Biotechnology Progress·Gyeongho SeonYong Keun Chang
Jul 19, 2019·Water Science and Technology : a Journal of the International Association on Water Pollution Research·William MichelonMárcio Luís Busi da Silva
Apr 1, 2020·International Journal of Environmental Research and Public Health·Xudan LuShulian Xie
Nov 13, 2018·Applied Biochemistry and Biotechnology·Rachidi FaridE L Arroussi Hicham
Sep 19, 2019·Environmental Technology·Duddela VaraprasadThummala Chandrasekhar
Jul 11, 2020·Journal, Genetic Engineering & Biotechnology·Mostafa El-SheekhWagdy Labib
Feb 23, 2021·Water Science and Technology : a Journal of the International Association on Water Pollution Research·Xiurong ChenQuanling Lu
Jan 21, 2021·International Journal of Biological Macromolecules·Jorge Lucas da MaiaMichele Greque de Morais
Jun 1, 2021·Journal of Environmental Management·C M CatoneS Ulgiati

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