Bioethanol production from uncooked raw starch by immobilized surface-engineered yeast cells

Applied Biochemistry and Biotechnology
Jyh-Ping ChenHideki Fukuda

Abstract

Surface-engineered yeast Saccharomyces cerevisiae codisplaying Rhizopus oryzae glucoamylase and Streptococcus bovis alpha-amylase on the cell surface was used for direct production of ethanol from uncooked raw starch. By using 50 g/L cells during batch fermentation, ethanol concentration could reach 53 g/L in 7 days. During repeated batch fermentation, the production of ethanol could be maintained for seven consecutive cycles. For cells immobilized in loofa sponge, the concentration of ethanol could reach 42 g/L in 3 days in a circulating packed-bed bioreactor. However, the production of ethanol stopped thereafter because of limited contact between cells and starch. The bioreactor could be operated for repeated batch production of ethanol, but ethanol concentration dropped to 55% of its initial value after five cycles because of a decrease in cell mass and cell viability in the bioreactor. Adding cells to the bioreactor could partially restore ethanol production to 75% of its initial value.

Citations

Jul 17, 2012·Applied Microbiology and Biotechnology·W H van ZylM J Viktor
Apr 22, 2010·Journal of Industrial Microbiology & Biotechnology·Peter M KilonzoMaurice A Bergougnou
Apr 17, 2012·Enzyme and Microbial Technology·Syun-ichi YamakawaAkihiko Kondo
Mar 29, 2014·Critical Reviews in Biotechnology·Johann F GörgensEugéne van Rensburg
Aug 8, 2013·Critical Reviews in Biotechnology·Duygu Ercan, Ali Demirci
Mar 30, 2019·Journal of the Science of Food and Agriculture·Ewelina Strąk-GraczykDorota Żyżelewicz

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