Biofabrication, structure and characterization of an amylopectin-based cyclic glucan

Food & Function
Chen ChenMing Miao

Abstract

A novel amylopectin-based cyclic architecture was fabricated, arising from microbial branching enzyme treated waxy rice starch. The recombinant enzyme had a molecular weight of 72.0 kDa, and exhibited optimum activity at pH 7.0 and 75 °C. During the cyclization reaction catalyzed by a branching enzyme, the molecular weight of amylopectin rapidly decreased for the initial 2 h, and then very slowly decreased, tapering off at approximately 1.8 × 105 g mol-1 at 12 h. The number of A-chain fractions greatly increased, whereas the percentage of B-chain fractions decreased after enzymatic modification, accompanied by more α-1, 6 linkage formation. The core ring structure as a glucoamylase-resistant fraction had a number-average degree of polymerization of 21, which was constructed by 19 glucose units linked with, 2 glucosyl stubs at the O-6-position of the cyclic glucan through α-1,4 and α-1,6 linkages. Similar to large-ring cyclodextrin with equal glucose units, this cyclic glucan had a cavity geometry with two-circular loops and short stubs in perpendicular planes. Moreover, this cyclic glucan could complex with iodine for the host-guest formation. These results revealed the potential application of the amylopectin-based cyclic gluc...Continue Reading

References

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Aug 28, 2002·The Journal of Biological Chemistry·Marta C AbadJames H Geiger
Mar 29, 2011·Journal of Agricultural and Food Chemistry·Augustin ScalbertDavid Wishart
Jan 28, 2014·International Journal of Biological Macromolecules·Huan JiangTao Zhang
Jun 11, 2014·Critical Reviews in Food Science and Nutrition·Ming MiaoZhengyu Jin
Jul 28, 2016·Organic & Biomolecular Chemistry·Khaleel I AssafWerner M Nau
Jul 25, 2017·Journal of Agricultural and Food Chemistry·Jie XiaoQingrong Huang
Sep 27, 2018·Journal of Agricultural and Food Chemistry·Bingjing ZhengDavid Julian McClements
Feb 26, 2019·Critical Reviews in Food Science and Nutrition·Lijun Sun, Ming Miao
Sep 1, 2018·Comprehensive Reviews in Food Science and Food Safety·Ming MiaoJames N BeMiller

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Methods Mentioned

BETA
transglycosylation
electrophoresis
NMR
isothermal titration calorimetry
X-ray

Software Mentioned

Accelrys Discovery Studio
Molekel
ASTRA
CHARMM

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