Biofilm Bridges Forming Structural Networks on Patterned Lubricant-Infused Surfaces

Advanced Science
Wenxi LeiThomas Schwartz

Abstract

Despite many decades of research, biofilm architecture and spreading mechanisms are still not clear because of the heterogenous 3D structure within biofilms. Here, patterned "slippery" lubricant-infused porous surfaces are utilized to study biofilm structure of Pseudomonas aeruginosa, Stenotrophomonas maltophilia, and Staphylococcus aureus. It is found that bacteria are able to spread over bacteria-repellent lubricant-infused regions by using a mechanism, termed "biofilm bridges". Here, it is demonstrated that bacteria use bridges to form interconnected networks between distant biofilm colonies. Detailed structure of bridges shows a spatial distribution of bacteria with an accumulation of respiratory active bacteria and biomass in the bridges. The core-shell structure of bridges formed by two-species mixed population is illustrated. It is demonstrated that eDNA and nutrients have a strong effect on biofilm bridges formation. Thus, it is believed that biofilm bridging is important to reveal the structure and communication within biofilms.

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Citations

Aug 5, 2020·Biomaterials Science·Xiaosong WeiXinge Zhang
Dec 17, 2020·Advanced Materials·Haijun CuiPavel A Levkin
May 18, 2021·Journal of Basic Microbiology·Viviana SvarcovaJarmila Pazlarova
Aug 28, 2021·International Journal of Molecular Sciences·Davide CampocciaCarla Renata Arciola

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Methods Mentioned

BETA
fluorescence microscopy
Fluorescence

Software Mentioned

ImageJ
OriginPro

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