Abstract
Mastitis poses a considerable threat to productivity and to animal welfare on modern dairy farms. However, the common way of antibiotic treatment does not always lead to a cure. Unsuccessful cures can, among other reasons, occur due to biofilm formation of the causative agent. This has attracted interest from researchers to introduce promising alternative therapeutic approaches, such as the use of beneficial lactic acid bacteria (LAB). In fact, using LAB for treating mastitis probably requires the formation of a beneficial biofilm by the probiotic bacteria. The present study investigated the ability of five LAB strains, selected on the basis of results from previous studies, to remove and to replace pathogenic biofilms in vitro. For this purpose, Staphylococcus (S.) aureus ATCC 12,600 and two strains-S. xylosus (35/07) and S. epidermidis (575/08)-belonging to the group of coagulase negative staphylococci (CNS) were allowed to form biofilms in a 96-well plate. Subsequently, the LAB were added to the well. The biofilm challenge was evaluated by scraping off and suspending the biofilm cells, followed by a plate count of serial dilutions using selective media. All the LAB strains successfully removed the staphylococcal biofilms. Ho...Continue Reading
References
Jun 20, 2000·Antimicrobial Agents and Chemotherapy·J N AnderlP S Stewart
Jan 24, 2007·Journal of Applied Microbiology·R TallonM C Urdaci
May 3, 2007·The Veterinary Quarterly·T HalasaH Hogeveen
Aug 6, 2008·The Journal of Dairy Research·Katja KlostermannWilliam Meaney
Aug 6, 2008·The Journal of Dairy Research·Fiona CrispieColin Hill
Oct 28, 2008·Veterinary Microbiology·B E GillespieS P Oliver
May 19, 2009·The Journal of Dairy Research·Christine BeecherLinda Giblin
Dec 7, 2010·Veterinary Microbiology·Emilia VarhimoKirsi Savijoki
Nov 15, 2011·The Journal of Dairy Research·Ignacio D FrolaCristina I Bogni
Aug 18, 2012·Journal of Applied Microbiology·M C Leccese TerrafC Silva
Mar 28, 2013·Folia Microbiologica·Tatsuro HagiMasaru Nomura
Oct 2, 2013·Veterinary Microbiology·Carme Plumed-FerrerAtte von Wright
Jun 17, 2014·Journal of Dairy Science·P R FryI Dohoo
May 13, 2015·International Journal of Food Microbiology·Mónica D Fernández RamírezMasja N Nierop Groot
May 7, 2016·PloS One·Mareike Klinger-StrobelOliwia Makarewicz
Sep 8, 2016·Veterinary Microbiology·Karin ArturssonJenny Schelin
Nov 12, 2016·Applied and Environmental Microbiology·Silvia GonzálezPilar García
Dec 13, 2016·Veterinary Microbiology·Sarah Schönborn, Volker Krömker
May 20, 2017·The Journal of Dairy Research·Sarah SchönbornVolker Krömker
Mar 19, 2018·AIMS Microbiology·Jonathan K WallisJan-Hendrik Paduch
Citations
May 18, 2020·Journal of Applied Microbiology·N ZaatoutM Kecha
Jun 25, 2020·Foods·Efstathios GiaourisFlorence Dubois-Brissonnet
Sep 20, 2020·Applied Microbiology and Biotechnology·Fazlurrahman KhanYoung-Mog Kim
Mar 13, 2020·Infection and Drug Resistance·Abolfazl BarzegariSepideh Zununi Vahed
Aug 14, 2019·Critical Reviews in Microbiology·Angeliki AngelopoulouR Paul Ross
May 25, 2021·Frontiers in Veterinary Science·Regitze Renee PedersenElin Jørgensen