Biofilm development and computational screening for new putative inhibitors of a homolog of the regulatory protein BrpA in Streptococcus dysgalactiae subsp. dysgalactiae

International Journal of Medical Microbiology : IJMM
Cinthia Alves-BarrocoIlda Santos-Sanches

Abstract

Streptococcus dysgalactiae subsp. dysgalactiae (SDSD), a Lancefield group C streptococci (GCS), is a frequent cause of bovine mastitis. This highly prevalent disease is the costliest in dairy industry. Adherence and biofilm production are important factors in streptoccocal pathogenesis. We have previously described the adhesion and internalization of SDSD isolates in human cells and now we describe the biofilm production capability of this bacterium. In this work we integrated microbiology, imaging and computational methods to evaluate the biofilm production capability of SDSD isolates; to assess the presence of biofilm regulatory protein BrpA homolog in the biofilm producers; and to predict a structural model of BrpA-like protein and its binding to putative inhibitors. Our results show that SDSD isolates form biofilms on abiotic surface such as glass (hydrophilic) and polystyrene (hydrophobic), with the strongest biofilm formation observed in glass. This ability was mainly associated with a proteinaceous extracellular matrix, confirmed by the dispersion of the biofilms after proteinase K and trypsin treatment. The biofilm formation in SDSD isolates was also confirmed by confocal laser scanning microscopy (CLSM) and scanning el...Continue Reading

Citations

Oct 31, 2020·Frontiers in Microbiology·Cinthia Alves-BarrocoPedro Viana Baptista
Jan 12, 2021·Frontiers in Microbiology·Cinthia Alves-BarrocoAlexandra R Fernandes
Mar 23, 2021·Journal of Medical Microbiology·Paulina GlajznerMagdalena Szemraj
Aug 3, 2021·Frontiers in Microbiology·Cinthia Alves-BarrocoIlda Santos-Sanches
Aug 8, 2021·Microorganisms·Tina KabelitzMarcus Fulde

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