Biofilm formation and antimicrobial sensitivity of lactobacilli contaminants from sugarcane-based fuel ethanol fermentation

Antonie van Leeuwenhoek
Marina de Toledo Ferraz DelliasSiu Mui Tsai

Abstract

Industrial ethanol fermentation is subject to bacterial contamination that causes significant economic losses in ethanol fuel plants. Chronic contamination has been associated with biofilms that are normally more resistant to antimicrobials and cleaning efforts than planktonic cells. In this study, contaminant species of Lactobacillus isolated from biofilms (source of sessile cells) and wine (source of planktonic cells) from industrial and pilot-scale fermentations were compared regarding their ability to form biofilms and their sensitivity to different antimicrobials. Fifty lactobacilli were isolated and the most abundant species were Lactobacillus casei, Lactobacillus fermentum and Lactobacillus plantarum. The majority of the isolates (87.8%) were able to produce biofilms in pure culture. The capability to form biofilms and sensitivity to virginiamycin, monensin and beta-acids from hops, showed inter- and intra-specific variability. In the pilot-scale fermentation, Lactobacillus brevis, L. casei and the majority of L. plantarum isolates were less sensitive to beta-acids than their counterparts from wine; L. brevis isolates from biofilms were also less sensitive to monensin when compared to the wine isolates. Biofilm formation...Continue Reading

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Citations

Mar 27, 2020·BMC Microbiology·Stacy MartinezNatalia Schlabritz-Loutsevitch
Jan 15, 2020·Journal of Environmental Management·Ana Flávia Rezende SilvaKonrad Koch

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Datasets Mentioned

BETA
KR135381
KR135403
MF191677
MF191703

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

Excel
CodonCode Aligner
BLAST
Decipher
SAS

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