Biofilm Formation and Quorum-Sensing-Molecule Production by Clinical Isolates of Serratia liquefaciens

Applied and Environmental Microbiology
Sara Remuzgo-MartínezJosé Ramos-Vivas

Abstract

Serratia spp. are opportunistic human pathogens responsible for an increasing number of nosocomial infections. However, little is known about the virulence factors and regulatory circuits that may enhance the establishment and long-term survival of Serratia liquefaciens in the hospital environment. In this study, two reporter strains, Chromobacterium violaceum CV026 and VIR24, and high-resolution triple-quadrupole liquid chromatography-mass spectrometry (LC-MS) were used to detect and to quantify N-acyl-homoserine lactone (AHL) quorum-sensing signals in 20 S. liquefaciens strains isolated from clinical samples. Only four of the strains produced sufficient amounts of AHLs to activate the sensors. Investigation of two of the positive strains by high-performance liquid chromatography (HPLC)-MS confirmed the presence of significant amounts of short-acyl-chain AHLs (N-butyryl-l-homoserine lactone [C4-HSL] and N-hexanoyl-l-homoserine lactone [C6-HSL]) in both strains, which exhibited a complex and strain-specific signal profile that included minor amounts of other short-acyl-chain AHLs (N-octanoyl-l-homoserine lactone [C8-HSL] and N-3-oxohexanoyl-l-homoserine lactone [OC6-HSL]) and long-acyl-chain (C10, C12, and C14) AHLs. No correla...Continue Reading

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Citations

Aug 23, 2018·PloS One·Itziar Chapartegui-GonzálezJosé Ramos-Vivas
May 28, 2019·Journal of Food Science·Milagros Liseth Castillo RiveraUelinton Manoel Pinto
Jul 10, 2020·Proceedings of the National Academy of Sciences of the United States of America·Eva GatoAstrid Pérez
Jun 22, 2019·Scientific Reports·José Ramos-VivasUNKNOWN ENTHERE Study Group, the Group for Study of Infection in Transplantation of the Spanish Society of Infectious Diseases and C
Oct 3, 2018·Frontiers in Cellular and Infection Microbiology·Celia MayerAna Otero
May 20, 2021·Proceedings of the National Academy of Sciences of the United States of America·Avaneesh V NarlaNed S Wingreen

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