Biofilm formation by Pseudomonas fluorescens WCS365: a role for LapD

Microbiology
Shannon M Hinsa, George A O'Toole

Abstract

A role for the outer-membrane-associated LapA protein in early biofilm formation by Pseudomonas fluorescens WCS365 has previously been shown. This paper reports that lapD, a gene located adjacent to the lapA gene, also plays a role in biofilm formation. A mutation in lapD results in a conditional biofilm defect in a static assay - this biofilm phenotype is exacerbated when biofilm formation is assayed in a flow-cell system. Furthermore, a lapD mutation shows a partial defect in the transition from reversible to irreversible attachment, consistent with an early role for the lapD gene product in biofilm formation. LapD is shown to be localized to the inner membrane of P. fluorescens. The data show decreased LapA associated with the cell surface, but no apparent change in cytoplasmic levels of this protein or lapA transcription, in a lapD mutant. A model is proposed wherein the role of LapD in biofilm formation is modulating the secretion of the LapA adhesin.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J A LeighG C Walker
Jul 2, 1998·Applied and Environmental Microbiology·A G Matthysse, S McMahan
Oct 31, 1998·Applied and Environmental Microbiology·M LiuG C Walker
Nov 5, 1999·Methods in Enzymology·B B ChristensenS Molin
Nov 5, 1999·Methods in Enzymology·G A O'TooleR Kolter
Oct 6, 2000·Annual Review of Microbiology·G O'TooleR Kolter
Dec 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·M E Davey, G A O'toole
Sep 15, 2001·FEMS Microbiology Letters·M Y GalperinE V Koonin
Dec 10, 2003·Current Opinion in Microbiology·Jeremy S WebbStaffan Kjelleberg
Jan 21, 2004·Molecular Microbiology·Lisa Friedman, Roberto Kolter
Oct 2, 2004·Molecular Microbiology·Begoña GarcíaIñigo Lasa
Oct 22, 2004·Contributions to Microbiology·Andreas ReisnerSøren Molin
Apr 9, 2005·Cellular and Molecular Life Sciences : CMLS·U Römling
May 17, 2005·Environmental Microbiology·Morten GjermansenTim Tolker-Nielsen
Jul 1, 1943·Journal of Bacteriology·C E Zobell

❮ Previous
Next ❯

Citations

Feb 17, 2009·Proceedings of the National Academy of Sciences of the United States of America·Peter D NewellGeorge A O'Toole
Mar 4, 2010·Biofouling·Yannick LequetteChristine Faille
Jul 6, 2006·Applied and Environmental Microbiology·Robert M Q ShanksGeorge A O'Toole
Jan 17, 2012·Applied and Environmental Microbiology·David LalaounaWafa Achouak
Jun 19, 2012·Journal of Bacteriology·Debashree ChatterjeeHolger Sondermann
Feb 10, 2011·PLoS Biology·Marcos V A S NavarroHolger Sondermann
Jan 25, 2014·ACS Nano·Sofiane El-Kirat-ChatelYves F Dufrêne
Oct 13, 2012·Annual Review of Cell and Developmental Biology·Chelsea D Boyd, George A O'Toole
Aug 5, 2015·Proceedings of the National Academy of Sciences of the United States of America·Daniel R SmithPaula I Watnick
Dec 17, 2009·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Fabienne QuilèsAnne Delille
Jun 20, 2007·Research in Microbiology·Fátima Yousef, Manuel Espinosa-Urgel
Feb 4, 2009·Journal of Applied Microbiology·N BernbomL Gram
May 23, 2012·Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology·Carly V RedelmanGregory G Anderson
May 18, 2012·Protein Science : a Publication of the Protein Society·Petya Violinova KrastevaHolger Sondermann
Jan 25, 2011·Microbial Biotechnology·Craig DanielsJuan L Ramos
Apr 1, 2014·Cochlear Implants International·Yael GoldfingerJona Kronenberg
May 26, 2010·FEMS Immunology and Medical Microbiology·Morten HarmsenTim Tolker-Nielsen
Jul 12, 2007·FEMS Microbiology Letters·Abdelhamid AbbasFergal O'Gara
Sep 12, 2008·FEMS Microbiology Letters·Fátima Yousef-CoronadoManuel Espinosa-Urgel
May 18, 2016·FEMS Microbiology Letters·Aroa López-SánchezFernando Govantes
Jul 9, 2005·Advances in Applied Microbiology·Katharine Kierek-Pearson, Ece Karatan
Apr 14, 2017·Journal of Basic Microbiology·Kulandaivelu VelmourouganeAnil Kumar Saxena
Nov 4, 2015·Journal of Bacteriology·Matthew R Parsek

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.