Biofilms and delayed healing - an in vitro evaluation of silver- and iodine-containing dressings and their effect on bacterial and human cells.

International Wound Journal
Katie A BourdillonBreda M Cullen

Abstract

This study investigated whether there are differences in the ability of wound dressings to modulate certain factors known to affect wound healing. A selection of antimicrobial dressings (AQUACEL® Ag Extra™ , AQUACEL® Ag+ Extra™ , IODOFLEX™ , ACTICOAT™ 7 and PROMOGRAN PRISMA™ matrix) were tested for their effect on both bacterial bioburden and human dermal fibroblasts. Some dressings underwent further evaluation for activity against Pseudomonas aeruginosa biofilms using a colony-drip flow reactor model. The ability of in vitro biofilms to produce proteases, and the effect of PROMOGRAN PRISMA matrix on such proteases, was also investigated. All antimicrobial dressings tested reduced vegetative bacterial load; however, only PROMOGRAN PRISMA matrix was able to significantly reduce biofilm populations (P = 0·01). Additionally, PROMOGRAN PRISMA matrix was the only dressing that did not inhibit dermal fibroblast growth. All other dressings were detrimental to cell viability. In vitro biofilms of Pseudomonas aeruginosa were demonstrated as being capable of releasing bacterial proteases into their surroundings, and incubation with PROMOGRAN PRISMA matrix led to a 77% reduction in activity of such proteases (P = 0·002). The unique abilit...Continue Reading

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Citations

Sep 12, 2018·Journal of Wound Care·Ruth ScullyPhilip Bowler
May 10, 2019·Tissue Engineering. Part B, Reviews·Mustafa Hussain KathawalaKee Woei Ng
May 19, 2021·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Briauna HawthorneAdam J Mellott
Jul 6, 2021·JAC-antimicrobial Resistance·Jean-Yves MaillardRose Cooper
Jul 29, 2021·Journal of Applied Microbiology·Andreea-Gabriela NedeleaSarah E Maddocks
Jul 2, 2021·Emerging Topics in Life Sciences·Davide Vincenzo VerdolinoSarah Cartmell

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