Biofilms Developed on Dental Implant Titanium Surfaces with Different Roughness: Comparison Between In Vitro and In Vivo Studies

Current Microbiology
Lorenzo BevilacquaLucilla Dolzani

Abstract

Microbial biofilms developed on dental implants play a major role in perimplantitis' pathogenesis. Many studies have indicated that surface roughness is the main feature favoring biofilm development in vitro, but its actual influence in vivo has still to be confirmed. In this study, the amount of biofilm formed on differently treated titanium surfaces, showing distinct roughness, has been examined both in vivo and in vitro by Confocal Laser Scanning Microscopy. In vitro studies availed of biofilm developed by Pseudomonas aeruginosa or by salivary bacteria from volunteer donors. In vivo biofilm production was obtained by exposing titanium discs to the oral cavity of healthy volunteers. In vitro experiments showed that P. aeruginosa and, to a lesser extent, salivary bacteria produce more biomass and develop thicker biofilms on laser-treated and sandblasted titanium surfaces with respect to machined ones. In vivo experiments confirmed that bacterial colonization starts on sites of surface unevenness, but failed to disclose biomass differences among biofilms formed on surfaces with different roughness. Our study revealed that biofilm developed in vitro is more easily influenced by surface features than biofilm formed by complex com...Continue Reading

References

May 1, 1987·Journal of Clinical Periodontology·J van DijkJ Arends
Sep 1, 1993·Clinical Oral Implants Research·M Quirynen, D van Steenberghe
Dec 13, 2000·Microbes and Infection·B Rosan, R J Lamont
Jun 12, 2001·Methods in Enzymology·R J PalmerP E Kolenbrander
Dec 12, 2001·Clinical Oral Implants Research·B Grössner-SchreiberU B Göbel
Nov 24, 2004·Colloids and Surfaces. B, Biointerfaces·Florence MabbouxOdile Barsotti
May 26, 2006·Journal of Dental Research·M M ShalabiN H J Creugers
Aug 15, 2006·Dental Materials : Official Publication of the Academy of Dental Materials·L Le GuéhennecY Amouriq
Sep 14, 2006·Clinical Oral Implants Research·Wim TeughelsMarc Quirynen
Sep 9, 2008·Journal of Clinical Periodontology·Jan LindheUNKNOWN Group D of European Workshop on Periodontology
Sep 9, 2008·Journal of Clinical Periodontology·Nicola U Zitzmann, Tord Berglundh
Aug 12, 2009·Clinical Oral Implants Research·Ann Wennerberg, Tomas Albrektsson
Nov 17, 2009·Clinical Oral Implants Research·Ralf BürgersMartin Gosau
Aug 21, 2010·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·A Al-AhmadR Kohal
Aug 29, 2013·Clinical Implant Dentistry and Related Research·Gordon JohnFrank Schwarz
Dec 7, 2013·Critical Reviews in Microbiology·Mira Okshevsky, Rikke Louise Meyer
Apr 12, 2014·Clinical Oral Implants Research·Matteo AlbertiniPedro Bullón
Apr 29, 2014·Archives of Oral Biology·I M G CavalcantiA A Del Bel Cury
Aug 12, 2014·Dental Materials : Official Publication of the Academy of Dental Materials·M C SánchezM Sanz
Dec 23, 2014·Journal of Prosthodontic Research·Yo Shibata, Yasuhiro Tanimoto
Sep 1, 2015·Journal of Molecular Biology·Morten RybtkeTim Tolker-Nielsen

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Citations

May 12, 2019·Clinical Oral Implants Research·Patricia BermejoMariano Sanz Alonso
Oct 23, 2019·Journal of Periodontal Research·Erica D de AvilaJeroen J J P van den Beucken
Jun 22, 2020·Clinical Oral Investigations·Matthias FolwacznyMadlena Betthäuser
Sep 6, 2018·Journal of Oral Microbiology·Inmaculada TomásCarlos Balsa-Castro
Jul 29, 2020·International Journal of Implant Dentistry·C MongardiniM Berardini
Aug 14, 2020·Lasers in Medical Science·Hebatallah MattarIman El Taftazany
Oct 17, 2018·International Journal of Molecular Sciences·Yu HaoLei Cheng
Oct 29, 2020·Clinical Oral Investigations·Torsten SterzenbachMatthias Hannig
Sep 4, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Jayanti MendhiYin Xiao
Oct 26, 2021·Frontiers in Cellular and Infection Microbiology·Jagjit Singh DhaliwalRubens Ferreira Albuquerque Junior

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Methods Mentioned

BETA
Assay
Scanning Microscopy

Software Mentioned

ImageJ
COMSTAT
CLSM

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