Biofloc-Based Enhanced Survival of Litopenaeus vannamei Upon AHPND-Causing Vibrio parahaemolyticus Challenge Is Partially Mediated by Reduced Expression of Its Virulence Genes

Frontiers in Microbiology
Vikash KumarPeter Bossier

Abstract

The biofloc system is a relatively new aquaculture technology that offers practical solution to maintain culture water quality by recycling nutrients and improves the health status and resistance of shrimps against microbial infection, yet the mode of action involved remains unclear. This study aimed to unravel the underlying mechanism behind the protective effect of a biofloc system using Litopenaeus vannamei and acute hepatopancreatic necrosis disease (AHPND)-causing Vibrio parahaemolyticus M0904 strain as a host-pathogen model. The results showed that a biofloc system maintained at a C/N ratio of 15, improves the water quality and contributes to the nutrition of cultured animals as bioflocs might serve as an additional protein source. Furthermore, the study demonstrated that the biofloc system enhances the survival of L. vannamei upon challenge with a V. parahaemolyticus AHPND strain. Remarkably, the results highlight that in the biofloc system, AHPND-causing V. parahaemolyticus possibly switch from free-living virulent planktonic phenotype to a non-virulent biofilm phenotype, as demonstrated by a decreased transcription of flagella-related motility genes (flaA, CheR, and fliS), Pir toxin (PirB VP ), and AHPND plasmid genes...Continue Reading

References

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Sep 25, 2017·Microbial Biotechnology·Peter Bossier, Julie Ekasari
Mar 25, 2018·Journal of Environmental Management·V Santhana KumarSaurav Kumar

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Citations

Aug 18, 2020·Emerging Microbes & Infections·Phuong Thi Ngoc TranPeter Bossier

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Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

geomean
GenStat

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