Bioflocculant exopolysaccharide production by Azotobacter indicus using flower extract of Madhuca latifolia L

Applied Biochemistry and Biotechnology
Satish Vitthalrao PatilBipinchandra K Salunke

Abstract

Efficacy of Azotobacter indicus ATCC 9540 strain for production exopolysaccharide (EPS) bioflocculant was investigated. Mahua flower extract (Madhuca latifolia L), a natural substrate at the concentration of 20 g L(-1), gave maximum recovery of EPS followed by sucrose and mannitol as compared to other carbon sources after 172 h. Yeast extract was found to be the most effective nitrogen source as compared to beef extract, sodium nitrate, ammonium sulfate, casein hydrolysate, and urea for the production of EPS. EPS production was increased in presence of nitrogen (5.51 g L(-1)) as compared to nitrogen-free medium (3.51 g L(-1)), and fermentation time was also reduced by 28 h. Maximum EPS production (6.10 g L(-1)) was found in the presence of 20 g L(-1) flower extract and 0.5 g L(-1) yeast extract containing Ashby's media with 180 rpm at 30 degrees C at 144 h, under controlled conditions in 2.5 L fermenter using optimized medium. The isolated EPS showed cation-dependent flocculating activity. Concentration of EPS played an important role in bioflocculating activity which increased in a concentration-dependent manner up to a certain limit, with the maximum flocculation of 72% at 500 mg L(-1) concentration but remained almost static...Continue Reading

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Citations

Aug 24, 2010·Applied Biochemistry and Biotechnology·Satish Vitthalrao PatilDeepak M Patil
Sep 23, 2010·Applied Biochemistry and Biotechnology·Satish Vitthalrao PatilR B Salunkhe
Feb 9, 2012·International Journal of Molecular Sciences·Leonard V MabinyaAnthony I Okoh
Jul 25, 2019·World Journal of Microbiology & Biotechnology·Jibrin Ndejiko Mohammed, Wan Rosmiza Zana Wan Dagang
Mar 8, 2020·Water Science and Technology : a Journal of the International Association on Water Pollution Research·Jibrin Ndejiko Mohammed, Wan Rosmiza Zana Wan Dagang
Mar 10, 2017·Preparative Biochemistry & Biotechnology·L MuthulakshmiFenila Christopher

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