Biofortification of soybean meal: immunological properties of the 27 kDa γ-zein

Journal of Agricultural and Food Chemistry
Hari B KrishnanHarold N Trick

Abstract

Legumes, including soybeans ( Glycine max ), are deficient in sulfur-containing amino acids, which are required for the optimal growth of monogastric animals. This deficiency can be overcome by expressing heterologous proteins rich in sulfur-containing amino acids in soybean seeds. A maize 27 kDa γ-zein, a cysteine-rich protein, has been successfully expressed in several crops including soybean, barley, and alfalfa with the intent to biofortify these crops for animal feed. Previous work has shown that the maize 27 kDa zein can withstand digestion by pepsin and elicit an immunogenic response in young pigs. By use of sera from patients who tested positive by ImmunoCAP assay for elevated IgE to maize proteins, specific IgE binding to the 27 kDa γ-zein is demonstrated. Bioinformatic analysis using the full-length and 80 amino acid sliding window FASTA searches identified significant sequence homology of the 27 kDa γ-zein with several known allergens. Immunoblot analysis using human serum that cross-reacts with maize seed proteins also revealed specific IgE-binding to the 27 kDa γ-zein in soybean seed protein extracts containing the 27 kDa zein. This study demonstrates for the first time the allergenicity potential of the 27 kDa γ-z...Continue Reading

References

Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W R Pearson, D J Lipman
Mar 14, 1996·The New England Journal of Medicine·J A NordleeR K Bush
Sep 18, 1998·Die Nahrung·K MüntzT Wüstenhagen
Oct 14, 2000·The Journal of Allergy and Clinical Immunology·E A PastorelloC Ortolani
Sep 10, 2002·International Archives of Allergy and Immunology·Ronald E HilemanSusan L Hefle
Sep 21, 2002·Lancet·Scott H Sicherer
Feb 8, 2003·Environmental Health Perspectives·Rhoda Sheryl Kagan
Apr 3, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Y ZhangP R Shewry
May 8, 2004·The Journal of Allergy and Clinical Immunology·Hugh A Sampson
Oct 27, 2004·Plant Physiology·Davide MainieriAlessandro Vitale
Jun 11, 2005·International Archives of Allergy and Immunology·Richard E GoodmanRonald van Ree
Sep 30, 2005·Journal of Agricultural and Food Chemistry·Sung-Ho LeeBruce R Hamaker
Jul 1, 1986·Plant Physiology·H B Krishnan, T W Okita
Jan 10, 2008·Nature Biotechnology·Richard E GoodmanRonald van Ree
Sep 10, 2008·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J ScibiliaC Ortolani
Nov 20, 2008·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·C VenterT Dean
Apr 16, 2009·Journal of Proteomics·Elisa FasoliPier Giorgio Righetti
Nov 1, 2005·Expert Review of Clinical Immunology·Richard E Goodman, Sue L Hefle
May 25, 2010·Journal of Agricultural and Food Chemistry·Hari B KrishnanChunjiang J Fu

❮ Previous
Next ❯

Citations

May 17, 2013·Journal of Agricultural and Food Chemistry·Hari B Krishnan, Ming-Hsuan Chen
Jan 25, 2012·Journal of Proteomics·Cátia FonsecaRita Batista
Oct 12, 2011·Current Opinion in Clinical Nutrition and Metabolic Care·Robert A McPherson, Gil Hardy
Mar 3, 2019·Plant Science : an International Journal of Experimental Plant Biology·Won-Seok Kim, Hari B Krishnan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.