Biofunctionalization of Nano Channels by Direct In-Pore Solid-Phase Peptide Synthesis

Chemistry : a European Journal
Martin BrodrechtGerd Buntkowsky

Abstract

Diatom biosilica are highly complex inorganic/organic hybrid materials. To get deeper insights on their structure at a molecular level, model systems that mimic the complex natural compounds were synthesized and characterized. A simple and efficient peptide immobilization strategy was developed, which uses a well-ordered porous silica material as a support and commercially available Fmoc-amino acids, similar to the known solid-phase peptide synthesis. As an example, Fmoc-glycine and Fmoc-phenylalanine are immobilized on the silica support. The success of functionalization was investigated by 13 C CP MAS and 29 Si CP MAS solid-state NMR. Thermogravimetric analysis (TGA) and elemental analysis (EA) were performed to quantify the functionalization. Changes of the specific surface area, pore volume, and pore diameters in all modification steps were studied by Brunauer-Emmett-Teller based nitrogen adsorption-desorption measurements (BET). The combination of the analytical methods provided high grafting densities of 2.1±0.2 molecules/nm2 on the surface. Furthermore, they allowed for monitoring chemical changes on the pore surface and changes of the pore properties of the material during the different functionalization steps. This uni...Continue Reading

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Citations

Feb 19, 2019·Chemistry : a European Journal·Martin BrodrechtGerd Buntkowsky
Jul 28, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Gerd Buntkowsky, Michael Vogel
Dec 31, 2020·Proceedings of the National Academy of Sciences of the United States of America·Alexandra NavrotskyAlbert Voskanyan
Mar 24, 2021·The Journal of Chemical Physics·Elisa SteinrückenMichael Vogel
May 25, 2021·Physical Chemistry Chemical Physics : PCCP·Marcos de OliveiraGerd Buntkowsky

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