Biofunctionalized cellulose paper matrix for cell delivery applications

International Journal of Biological Macromolecules
Tarun AgarwalManchikanti Padmavati

Abstract

The present study delineates the preparation, characterization, and application of (3-Aminopropyl)triethoxysilane (APTES)/Caprine liver-derived extracellular matrix (CLECM) coated paper matrix for cell delivery. Here, we exploited positively charged surface of the paper matrix (as imparted by APTES derivatization) to improve the biological responses of the cells. Our results demonstrated that the functionalized paper matrixes favored the adhesion, growth, and proliferation of multiple cell types including normal, transformed, cancerous, and stem cells as compared to the pristine paper matrix. Upon implantation into the mice model, the developed paper matrix supported infiltration of the host cells and vasculature without showing any evidence of significant systemic toxicity. Moreover, the cells cultured on the paper matrix, when delivered to the CAM and mouse models, showed an enhanced vascular network around the substrate, thereby confirming its potential to deliver the cells in vivo. Together, the study confirms that the reported paper-based platform is easy to fabricate, cheap, portable and could efficiently be applied to cell delivery applications for either tissue repair or the development of humanized animal model.

References

Feb 9, 2002·Science·Linda G Griffith, Gail Naughton
Aug 23, 2006·Cell·Adam J EnglerDennis E Discher
Nov 21, 2007·Current Opinion in Biotechnology·R Warren Sands, David J Mooney
Mar 29, 2008·Cell Stem Cell·David J Mooney, Herman Vandenburgh
Oct 24, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Robert M PasternackYves J Chabal
Jun 6, 2009·Tissue Engineering. Part B, Reviews·Michael LovettDavid L Kaplan
Oct 23, 2009·Proceedings of the National Academy of Sciences of the United States of America·Ratmir DerdaGeorge M Whitesides
Dec 17, 2009·Analytical Chemistry·Andres W MartinezEmanuel Carrilho
May 17, 2011·PloS One·Ratmir DerdaGeorge M Whitesides
Jul 13, 2011·Proceedings of the National Academy of Sciences of the United States of America·Alice A ChenSangeeta N Bhatia
Aug 16, 2011·Biosensors & Bioelectronics·Ya-Hsuan ChuangTri-Rung Yew
Apr 23, 2013·Analytical and Bioanalytical Chemistry·Emilia W Nery, Lauro T Kubota
Feb 28, 2014·Advanced Healthcare Materials·Bobak MosadeghGeorge M Whitesides
Sep 23, 2014·Biomaterials·Hyun-Ji ParkSeung-Woo Cho
Nov 10, 2015·The Analyst·Rachael M KenneyMatthew R Lockett
Nov 20, 2015·Physical Chemistry Chemical Physics : PCCP·Chia-Chi ChangMasaki Ujihara
Dec 2, 2015·Proceedings of the National Academy of Sciences of the United States of America·Su-Hwan KimNathaniel S Hwang
Dec 17, 2015·Analytical and Bioanalytical Chemistry·Matthew W BoyceMatthew R Lockett
Jul 6, 2016·Lab on a Chip·Chia-Hao HuangNgan-Ming Tsang
Mar 30, 2017·ACS Applied Materials & Interfaces·Kin Fong LeiMing-Jie Chen
May 24, 2017·Colloids and Surfaces. B, Biointerfaces·N S Kehr, A Motealleh
Oct 17, 2017·Colloids and Surfaces. B, Biointerfaces·Rajan NarayanTapas Kumar Maiti
Mar 15, 2018·Frontiers in Neuroscience·Mathias J AebersoldJános Vörös
Sep 5, 2018·Colloids and Surfaces. B, Biointerfaces·Tarun AgarwalTapas Kumar Maiti
Feb 20, 2019·Biomaterials Science·Christopher M Jewell, Joel H Collier
Mar 1, 2019·Materials Science & Engineering. C, Materials for Biological Applications·Tarun AgarwalSudip Kumar Ghosh
Mar 26, 2019·ACS Applied Materials & Interfaces·Min WangZhongze Gu

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Citations

Aug 24, 2021·Biomacromolecules·Paula De Dios AndresBrigitte Städler

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