Biogenesis of Candida albicans Can1 permease expressed in Saccharomyces cerevisiae

FEBS Letters
A Matìjèková, H Sychrová

Abstract

The Candida albicans CAN1 gene, encoding a high-affinity permease for arginine, lysine and histidine, was tagged at its C-terminus with a c-myc epitope and introduced into strains of Saccharomyces cerevisiae lacking basic amino acid permeases. The expression levels of Ca-Can1p were influenced by the available nitrogen source, being almost negligible when cells were grown in the presence of ammonia. Ca-Can1p was shown to follow the secretory pathway in S. cerevisiae. Ca-Can1p activity was not detected in a secretion-defective sec1-1 mutant grown at a non-permissive temperature. Shr3p, an ER protein that participates in the biogenesis of amino acid permeases was also required for the functional expression of Ca-Can1p. The shr3 mutation does not affect the affinity for substrate but does decrease the number of Can1p molecules reaching the plasma membrane.

References

Jan 1, 1992·Annual Review of Biochemistry·N K PryerR Schekman
Feb 1, 1991·Molecular and Cellular Biology·S SilveR Haguenauer-Tsapis
Apr 8, 1985·FEBS Letters·H Sychrová, A Kotyk
Dec 22, 1986·Biochimica Et Biophysica Acta·J Horák
Feb 1, 1993·Current Genetics·P ReisdorfB Daignan-Fornier
Jan 1, 1997·Folia Microbiologica·A Matĕjcková, H Sychrová

❮ Previous
Next ❯

Citations

Jun 21, 2002·Biochemical and Biophysical Research Communications·Blanka ZikánováZdena Palková
Jul 27, 1999·FEMS Microbiology Letters·A Matĕjcková-ForejtováH Sychrová
Jul 25, 2018·Nature Microbiology·Katharina TrunkSarah J Coulthurst
Mar 15, 2006·Fungal Genetics and Biology : FG & B·Z ErpapazoglouV Sophianopoulou

❮ Previous
Next ❯

Related Concepts

Related Feeds

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.