Biogenesis of Epstein-Barr virus microRNAs.

Molecular and Cellular Biochemistry
Do Nyun Kim, Suk Kyeong Lee

Abstract

Epstein-Barr virus (EBV) is a ubiquitous human herpesvirus implicated in lymphomas, such as Burkitt's lymphoma, Hodgkin's lymphoma, and NK/T cell lymphoma. MicroRNAs (miRNAs) are 19-25 nucleotide long single-stranded RNAs involved in post-transcriptional gene regulation. miRNAs are mainly transcribed by RNA polymerase II (pol II) to have stem-loop structures and subsequently processed by Drosha and Dicer. EBV miRNAs are expressed in B cells, nasopharyngeal carcinoma cells, and gastric carcinoma cells infected with EBV. EBV miRNAs can be divided into two groups: BHRF1 miRNAs and BART miRNAs. In this study, we investigated the biogenesis of EBV miRNAs. Treatment of the SNU-719 EBV-positive gastric cancer cell line with α-amanitin at a concentration that selectively inhibits RNA polymerase II activity decreased the expression levels of BART miRNAs. The expression levels of BART miRNAs were also reduced by RNA interference targeting Drosha and Dicer. Two of each C/EBPβ and c-Myc binding sites are located upstream of the proposed initiation sites for primary BART miRNA transcripts. Knockdown of C/EBPβ but not c-Myc using siRNAs reduced BART miRNA expression by 25-55% compared with the control. These results suggest that BART miRNAs ...Continue Reading

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Citations

Dec 3, 2013·Asian Pacific Journal of Cancer Prevention : APJCP·Yuan-Jie XieXiu-Sheng He
Apr 27, 2013·International Reviews of Immunology·Leandra Fiori LopesMaria Angelica Ehara Watanabe
Apr 14, 2015·Future Microbiology·Farshid S GarmaroudiBruce M McManus
Mar 16, 2016·Oxidative Medicine and Cellular Longevity·Aldo GiudiceMaurizio Montella
Nov 12, 2014·Cancers·Jun NishikawaIsao Sakaida
Jan 31, 2015·International Journal of Oncology·Aya Shinozaki-UshikuMasashi Fukayama
Jul 3, 2017·Virologica Sinica·Lielian ZuoJianhong Lu
Apr 21, 2018·Frontiers in Microbiology·Iva PolakovicovaAlejandro H Corvalán
Nov 29, 2020·Cancer Letters·Brunno Felipe R CaetanoDeilson Elgui de Oliveira

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