PMID: 1202019Dec 1, 1975Paper

Biogenesis of microsomal membrane glycoproteins in rat liver. II. Purification of soluble glycoproteins and their incorporation into microsomal membranes

The Journal of Cell Biology
F AutuoriG Dallner

Abstract

Sialoproteins isolated from the soluble fraction of rat liver could be incorporated into microsomal membranes. This incorporation was dependent on protein concentration, time, and temperature. Sodium dodecyl sulfate gel electrophoresis of membrane proteins after in vitro incorporation showed four major sugar-containing peaks and was similar to that found after in vivo labeling. Most of the incorporated protein was tightly bound to the microsomal membrane. Gel filtration and ion-exchange chromatography revealed the presence of several cytosolic glycoproteins that could be incorporated into microsomes. During prolonged centrifugation in a KBr solution with a density of 1.21 a highly labeled ([3H]glucosamine) protein (mole wt approximately to 70,000) that was actively incorporated into microsomes could be recovered in the upper region of the tube. These results demonstrate that several cytoplasmic glycoproteins of rat liver are transferred into microsomal membranes and that one of these is a lipoprotein.

References

Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·G Assmann, H B Brewer
Jul 24, 1974·Biochemical and Biophysical Research Communications·J B SwaneyH A Eder
Jan 1, 1970·Annual Review of Biochemistry·R T Schimke, D Doyle
Mar 1, 1969·Archives of Biochemistry and Biophysics·H B BosmannE H Eylar

❮ Previous
Next ❯

Citations

Sep 1, 1978·European Journal of Biochemistry·O S NilssonF W Hemming
Apr 1, 1978·FEBS Letters·J A CraftB R Rabin
May 15, 1979·European Journal of Biochemistry·J A CraftB R Rabin
Apr 20, 1978·Biochimica Et Biophysica Acta·G Gogstad, L Helgeland
Sep 11, 1978·Biochimica Et Biophysica Acta·E L AppelkvistG Dallner
Aug 9, 1977·Biochimica Et Biophysica Acta·G C Shore, J R Tata
Apr 23, 1979·Biochimica Et Biophysica Acta·D J MorréW W Franke

❮ Previous
Next ❯

Related Concepts

Related Feeds

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.