Biogenesis of phycobiliproteins: II. CpcS-I and CpcU comprise the heterodimeric bilin lyase that attaches phycocyanobilin to CYS-82 OF beta-phycocyanin and CYS-81 of allophycocyanin subunits in Synechococcus sp. PCC 7002

The Journal of Biological Chemistry
Nicolle A SaunéeWendy M Schluchter

Abstract

The Synechococcus sp. PCC 7002 genome encodes three genes, denoted cpcS-I, cpcU, cpcV, with sequence similarity to cpeS. CpcS-I copurified with His(6)-tagged (HT) CpcU as a heterodimer, CpcSU. When CpcSU was assayed for bilin lyase activity in vitro with phycocyanobilin (PCB) and apophycocyanin, the reaction product had an absorbance maximum of 622 nm and was highly fluorescent (lambda(max) = 643 nm). In control reactions with PCB and apophycocyanin, the products had absorption maxima at 635 nm and very low fluorescence yields, indicating they contained the more oxidized mesobiliverdin (Arciero, D. M., Bryant, D. A., and Glazer, A. N. (1988) J. Biol. Chem. 263, 18343-18349). Tryptic peptide mapping showed that the CpcSU-dependent reaction product had one major PCB-containing peptide that contained the PCB binding site Cys-82. The CpcSU lyase was also tested with recombinant apoHT-allophycocyanin (aporHT-AP) and PCB in vitro. AporHT-AP formed an ApcA/ApcB heterodimer with an apparent mass of approximately 27 kDa. When aporHT-AP was incubated with PCB and CpcSU, the product had an absorbance maximum of 614 nm and a fluorescence emission maximum at 636 nm, the expected maxima for monomeric holo-AP. When no enzyme or CpcS-I or CpcU...Continue Reading

References

Aug 14, 1992·Journal of Photochemistry and Photobiology. B, Biology·Y M GindtK Sauer
Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C D FairchildA N Glazer
Aug 9, 1990·Biochimica Et Biophysica Acta·R de LorimierS E Stevens
Oct 26, 1989·Biochimica Et Biophysica Acta·P MaxsonA N Glazer
Jan 1, 1988·Methods in Enzymology·A N Glazer
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R de LorimierS E Stevens
Feb 1, 1997·Journal of Bacteriology·K KahnM R Schaefer
Jan 7, 2003·The Journal of Biological Chemistry·Nicole Frankenberg, J Clark Lagarias
Dec 29, 2004·Biochimica Et Biophysica Acta·Kai-Hong ZhaoHugo Scheer
Aug 30, 2007·Proceedings of the National Academy of Sciences of the United States of America·Kai-Hong ZhaoHugo Scheer

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Nov 24, 2012·Nucleic Acids Research·Anthony BretaudeauFrédéric Partensky
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