PMID: 2110571May 1, 1990Paper

Biogenesis of synaptic vesicle-like structures in a pheochromocytoma cell line PC-12

The Journal of Cell Biology
L Clift-O'GradyR B Kelly

Abstract

The presence of unique proteins in synaptic vesicles of neurons suggests selective targeting during vesicle formation. Endocrine, but not other cells, also express synaptic vesicle membrane proteins and target them selectively to small intracellular vesicles. We show that the rat pheochromocytoma cell line, PC12, has a population of small vesicles with sedimentation and density properties very similar to those of rat brain synaptic vesicles. When synaptophysin is expressed in nonneuronal cells, it is found in intracellular organelles that are not the size of synaptic vesicles. The major protein in the small vesicles isolated from PC12 cells is found to be synaptophysin, which is also the major protein in rat brain vesicles. At least two of the minor proteins in the small vesicles are also known synaptic vesicle membrane proteins. Synaptic vesicle-like structures in PC12 cells can be shown to take up an exogenous bulk phase marker, HRP. Their proteins, including synaptophysin, are labeled if the cells are surface labeled and subsequently warmed. Although the PC12 vesicles can arise by endocytosis, they seem to exclude the receptor-mediated endocytosis marker, transferrin. We conclude that PC12 cells contain synaptic vesicle-like...Continue Reading

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Citations

Dec 15, 1996·Journal of Neuroscience Research·J P BresslerG W Goldstein
Mar 1, 1997·Journal of Neuroscience Research·P RedeckerS Steinlechner
May 1, 1992·Journal of Cellular Biochemistry·A ShalevM Linial
Feb 1, 1993·Journal of Neurocytology·J J Lah, R W Burry
Jan 1, 1996·Behavioural Brain Research·Y LiuR H Edwards
Aug 1, 1991·Current Opinion in Cell Biology·R B Kelly
Jun 1, 1991·Current Opinion in Neurobiology·M Matteoli, P De Camilli
Oct 1, 1991·Current Opinion in Neurobiology·W B Huttner, C G Dotti
Jul 2, 2002·Journal of Neurochemistry·Arjan GeerlingsCarmen Aragón
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A Thomas-ReetzP De Camilli
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·G A HermanR B Kelly
Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·J van de GoorR Kelly
Mar 1, 1991·The Journal of Cell Biology·L Matsuuchi, R B Kelly
Nov 1, 1991·The Journal of Cell Biology·M MatteoliP De Camilli
Feb 1, 1992·The Journal of Cell Biology·M K BennettR H Scheller
Dec 1, 1994·The Journal of Cell Biology·R E LeubeB Wiedenmann
Sep 1, 1995·The Journal of Cell Biology·C DesnosR B Kelly
Dec 1, 1995·The Journal of Cell Biology·K SteniusR Jahn
Feb 1, 1996·The Journal of Cell Biology·E Grote, R B Kelly
Jun 1, 1996·The Journal of Cell Biology·R A ChavezH P Moore
Sep 1, 1996·The Journal of Cell Biology·J P NorcottD F Cutler
Nov 1, 1996·The Journal of Cell Biology·J H Koenig, K Ikeda
Dec 10, 1999·Molecular Biology of the Cell·H de WitJ Klumperman
Nov 6, 2001·Molecular Biology of the Cell·H de WitP van der Sluijs
Jun 10, 2005·Molecular Biology of the Cell·Gloria SalazarVictor Faundez

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