PMID: 6397228Dec 18, 1984Paper

Biogenesis, transit, and functional properties of the insulin proreceptor and modified insulin receptors in 3T3-L1 adipocytes. Use of monensin to probe proreceptor cleavage and generate altered receptor subunits

Biochemistry
A SalzmanC S Rubin

Abstract

The biogenesis, intracellular transport, and functional properties of the insulin proreceptor and modified insulin receptors were studied in hormone-responsive 3T3-L1 adipocytes. After control cells were labeled with [35S]Met for 7 min, the principal polypeptide that was precipitated by anti-insulin receptor antibodies had a molecular weight (Mr) of 180 000. This initial precursor was rapidly converted (t1/2 = 35 min) to a 200-kilodalton (kDa) polypeptide, designated the insulin proreceptor, by the apparent posttranslational addition of N-linked, high mannose core oligosaccharide units. Mature alpha (Mr 130 000) and beta (Mr 90 000) subunits were derived from sequences within the proreceptor by proteolytic cleavage and late processing steps, and these subunits appeared on the cell surface 2-3 h after synthesis of the 180-kDa precursor. The cation ionophore monensin was used in combination with metabolic labeling, affinity cross-linking, and external proteolysis to probe aspects of proreceptor function, transit, and the development of insulin sensitivity at the target cell surface. At 5 micrograms/mL, monensin potently inhibited the proteolytic cleavage step, and the 200-kDa polypeptide accumulated. Lower concentrations of the i...Continue Reading

References

Dec 1, 1979·Cell·M S KrangelJ L Strominger
Dec 1, 1978·The Journal of Cell Biology·A TartakoffM Détraz
Nov 1, 1979·The Journal of Cell Biology·A Tartakoff, P Vassalli
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·S JacobsP Cuatrecasas
Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·B C ReedM D Lane
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·N UchidaM L Tanzer
Apr 1, 1979·Journal of Cellular Physiology·O M RosenC S Rubin
Jun 16, 1978·Science·S JacobsP Cuatrecasas
Aug 8, 1977·Biochemical and Biophysical Research Communications·S JacobsP Cuatrecasas
Nov 1, 1977·The Journal of Experimental Medicine·A M Tartakoff, P Vassalli
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·L M PetruzzelliO M Rosen
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S JacobsP Cuatrecasas
Feb 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·E Van ObberghenG Ponzio
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·P J DeutschC S Rubin
Aug 25, 1981·Journal of Molecular Biology·A TartakoffP Vassalli
Jan 21, 1983·Science·R A Roth, D J Cassell
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J MassagueM P Czech
Feb 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·E Van ObberghenL C Harrison
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·B C ReedM D Lane
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J A HedoC R Kahn
Jan 29, 1980·Biochemical and Biophysical Research Communications·M H WisherP H Sönksen
Jan 1, 1981·Annual Review of Biochemistry·S C Hubbard, R J Ivatt
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M KasugaK M Yamada
Sep 11, 1981·Science·J E Rothman

❮ Previous
Next ❯

Citations

Jan 1, 1986·The Journal of Clinical Investigation·C F BurantM G Buse
Apr 14, 1989·Biochemical and Biophysical Research Communications·R F ArakakiP Gorden
Jun 24, 2015·PLoS Genetics·Rachel D StarksKamal Rahmouni
Dec 18, 2002·Journal of Neurochemistry·Masato InazuTeruhiko Matsumiya
Jul 18, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shiqun LiuMing Liu
Jul 11, 2020·The Biochemical Journal·Yasuhiro OnogiSiegfried Ussar
Nov 1, 1991·The American Journal of Physiology·D DardevetM G Buse
Feb 1, 1994·The American Journal of Physiology·J Lee, P F Pilch
Aug 1, 1987·Molecular and Cellular Biology·R Fernandez-Almonacid, O M Rosen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.