Biogeographic Comparison of Lophelia-Associated Bacterial Communities in the Western Atlantic Reveals Conserved Core Microbiome

Frontiers in Microbiology
Christina A KelloggMichael A Gray

Abstract

Over the last decade, publications on deep-sea corals have tripled. Most attention has been paid to Lophelia pertusa, a globally distributed scleractinian coral that creates critical three-dimensional habitat in the deep ocean. The bacterial community associated with L. pertusa has been previously described by a number of studies at sites in the Mediterranean Sea, Norwegian fjords, off Great Britain, and in the Gulf of Mexico (GOM). However, use of different methodologies prevents direct comparisons in most cases. Our objectives were to address intra-regional variation and to identify any conserved bacterial core community. We collected samples from three distinct colonies of L. pertusa at each of four locations within the western Atlantic: three sites within the GOM and one off the east coast of the United States. Amplicon libraries of 16S rRNA genes were generated using primers targeting the V4-V5 hypervariable region and 454 pyrosequencing. The dominant phylum was Proteobacteria (75-96%). At the family level, 80-95% of each sample was comprised of five groups: Pirellulaceae, Pseudonocardiaceae, Rhodobacteraceae, Sphingomonadaceae, and unclassified Oceanospirillales. Principal coordinate analysis based on weighted UniFrac dis...Continue Reading

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Citations

Mar 15, 2018·Journal of Toxicology and Environmental Health. Part a·Thierry BaussantSreerekha Ramanand
Apr 25, 2018·Environmental Microbiology·Varsha MathurPatrick J Keeling
Mar 6, 2019·Environmental Microbiology Reports·Alexander T NeuKaustuv Roy
Jun 19, 2019·FEMS Microbiology Ecology·Sigmund JensenDavid G Bourne
Sep 14, 2018·Frontiers in Microbiology·Jacquelyn JonesMegan J Huggett
May 15, 2018·Frontiers in Microbiology·Deborah C A LeiteRaquel S Peixoto
Nov 7, 2019·Environmental Microbiology·Pierre E GalandFranck Lartaud
Mar 13, 2020·Scientific Reports·Jinlong SongYingchun Mu

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Datasets Mentioned

BETA
PRJNA305617

Methods Mentioned

BETA
Assay
454 sequencing

Software Mentioned

QIIME Amazon Machine Image ( AMI )
R
vegan
SIMPER
ClustalX
uclust
mothur
QIIME
PICRUSt
- E Analysis of Similarities ( ANOSIM )

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