Bioimaging of dexamethasone and TGF beta-1 and its biological activities of chondrogenic differentiation in hydrogel constructs

Journal of Biomedical Materials Research. Part a
Kun NaKeun Hong Park

Abstract

This study examined the efficacy of poly(NiPAAm-co-AAc) as an injectable drug delivery vehicle and a cell therapeutic agent in the form of a supporting matrix for the chondrogenic differentiation of rabbit chondrocytes. The hydrogel constructs, which consisted of embedded cells co-encapsulating dexamethasone (Dex) and TGF beta-1 or unloaded Dex, were used as controls to determine the effects of Dex and TGF beta-1 on chondrogenic differentiation. The level of Dex and TGF beta-1 released was monitored using a bioimaging method. The amount of Dex released from hydrogel was faster than that of TGF beta-1. TGF beta-1 was present in hydrogel for more than 4 weeks after the injection. The level of the cartilage associated ECM proteins was examined by immunohistochemical staining for collagen type II as well as by Safranin-O and Alcian blue (GAG) staining. These results highlight the potential of a thermo-reversible hydrogel mixed with the chondrocytes and differentiation delivery material for applications in neocartilage formation.

References

Oct 1, 1988·Experimental Cell Research·W E HortonJ R Hassell
Jan 26, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M C StewartJ N Macleod
May 26, 2001·The Journal of Endocrinology·I SekiyaM Noda
Aug 13, 2004·Journal of Biomedical Materials Research. Part a·Mark A Rice, Kristi S Anseth
Sep 3, 2005·Journal of Biomedical Materials Research. Part a·Shinichi TeradaJoseph P Vacanti

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Citations

Jul 27, 2012·Journal of Biomaterials Applications·Liese N Beenken-RothkopfAnnelise E Barron
Jan 29, 2016·Annals of Biomedical Engineering·Joseph M MansourJean F Welter

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